The myosin interacting-heads motif present in live tarantula muscle explains tetanic and posttetanic phosphorylation mechanisms

^aDivision of Cell Biology and Imaging, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655;
^bBiophysics Collaborative Access Team, Department of Biological Sciences, Illinois Institute of Technology, Chicago, IL 60616;
^cCentro de Biología Estructural, Instituto Venezolano de Investigaciones Científicas, Caracas 1020A, Venezuela;
^dInstitute of Mechanics, Moscow State University, 119992 Moscow, Russia;
^eCentro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, Caracas 1020A, Venezuela

Contributed by Raúl Padrón, April 7, 2020 (sent for review December 6, 2019; reviewed by H. Lee Sweeney and Rene Vandenboom)

Footnotes

↵¹R.P. and W.M. contributed equally to this work.
↵²To whom correspondence may be addressed. Email: raul.padron{at}umassmed.edu.
↵³Department of Molecular Physiology and Biophysics, Cardiovascular Research Institute, University of Vermont, Burlington, VT 05405.
↵⁴Massachusetts Facility for High-Resolution Electron Cryo-Microscopy, University of Massachusetts Medical School, Worcester, MA 01655.

This contribution is part of the special series of Inaugural Articles by members of the National Academy of Sciences elected in 2018.
Author contributions: R.P., T.I., and R.C. designed research; R.P., W.M., S.D.-M., N.K., K.H.L., A.P., L.A., P.B., A.T., T.I., and R.C. performed research; R.P., W.M., S.D.-M., N.K., A.P., L.A., P.B., A.T., T.I., and R.C. analyzed data; and R.P., W.M., T.I., and R.C. wrote the paper.
Reviewers: H.S., University of Florida; and R.V., Brock University.
The authors declare no competing interest.
Data deposition: Data for this paper are available in the Open Science Framework repository at https://osf.io/anxuy/?view_only=5491c6ba8bca4add9d97930614734e0e.
This article contains supporting information online at https://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1921312117/-/DCSupplemental.