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Research Article

Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit

View ORCID ProfileYunxiao Zhang, View ORCID ProfileWan-Jin Lu, David P. Bulkley, Jiahao Liang, Arthur Ralko, Shuo Han, Kelsey J. Roberts, Anping Li, Wonhwa Cho, Yifan Cheng, View ORCID ProfileAashish Manglik, and Philip A. Beachy
PNAS November 17, 2020 117 (46) 28838-28846; first published November 2, 2020; https://doi.org/10.1073/pnas.2011560117
Yunxiao Zhang
aInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305;
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  • ORCID record for Yunxiao Zhang
Wan-Jin Lu
aInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305;
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  • ORCID record for Wan-Jin Lu
David P. Bulkley
bDepartment of Biochemistry and Biophysics, University of California, San Francisco, CA 94158;
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Jiahao Liang
cDepartment of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158;
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Arthur Ralko
dDepartment of Chemistry, University of Illinois at Chicago, Chicago, IL 60607;
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Shuo Han
aInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305;
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Kelsey J. Roberts
eDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305;
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Anping Li
aInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305;
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Wonhwa Cho
dDepartment of Chemistry, University of Illinois at Chicago, Chicago, IL 60607;
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Yifan Cheng
bDepartment of Biochemistry and Biophysics, University of California, San Francisco, CA 94158;
fHoward Hughes Medical Institute, University of California, San Francisco, CA 94158;
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Aashish Manglik
cDepartment of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158;
gDepartment of Anesthesia and Perioperative Care, University of California, San Francisco, CA 94158;
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  • For correspondence: aashish.manglik@ucsf.edu pbeachy@stanford.edu
Philip A. Beachy
aInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305;
eDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305;
hDepartment of Urology, Stanford University School of Medicine, Stanford, CA 94305;
iDepartment of Chemical and Systems Biology (by courtesy), Stanford University School of Medicine, Stanford, 94305
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  • For correspondence: aashish.manglik@ucsf.edu pbeachy@stanford.edu
  1. Contributed by Philip A. Beachy, September 23, 2020 (sent for review June 5, 2020; reviewed by James Briscoe and Volodymyr M. Korkhov)

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Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit
Yunxiao Zhang, Wan-Jin Lu, David P. Bulkley, Jiahao Liang, Arthur Ralko, Shuo Han, Kelsey J. Roberts, Anping Li, Wonhwa Cho, Yifan Cheng, Aashish Manglik, Philip A. Beachy
Proceedings of the National Academy of Sciences Nov 2020, 117 (46) 28838-28846; DOI: 10.1073/pnas.2011560117

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Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit
Yunxiao Zhang, Wan-Jin Lu, David P. Bulkley, Jiahao Liang, Arthur Ralko, Shuo Han, Kelsey J. Roberts, Anping Li, Wonhwa Cho, Yifan Cheng, Aashish Manglik, Philip A. Beachy
Proceedings of the National Academy of Sciences Nov 2020, 117 (46) 28838-28846; DOI: 10.1073/pnas.2011560117
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Proceedings of the National Academy of Sciences: 117 (46)
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