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Research Article

Natural cystatin C fragments inhibit GPR15-mediated HIV and SIV infection without interfering with GPR15L signaling

View ORCID ProfileManuel Hayn, Andrea Blötz, View ORCID ProfileArmando Rodríguez, View ORCID ProfileSolange Vidal, Nico Preising, View ORCID ProfileLudger Ständker, View ORCID ProfileSebastian Wiese, Christina M. Stürzel, Mirja Harms, View ORCID ProfileRüdiger Gross, View ORCID ProfileChristoph Jung, Miriam Kiene, View ORCID ProfileTimo Jacob, View ORCID ProfileStefan Pöhlmann, Wolf-Georg Forssmann, View ORCID ProfileJan Münch, View ORCID ProfileKonstantin M. J. Sparrer, View ORCID ProfileKlaus Seuwen, View ORCID ProfileBeatrice H. Hahn, and View ORCID ProfileFrank Kirchhoff
PNAS January 19, 2021 118 (3) e2023776118; https://doi.org/10.1073/pnas.2023776118
Manuel Hayn
aInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;
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  • ORCID record for Manuel Hayn
Andrea Blötz
aInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;
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Armando Rodríguez
bCore Facility Functional Peptidomics, Ulm University Medical Center, 89081 Ulm, Germany;
cCore Unit Mass Spectrometry and Proteomics, Ulm University Medical Center, 89081 Ulm, Germany;
dPHARIS Biotec GmbH, 30625 Hannover, Germany;
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Solange Vidal
eNovartis Institutes for Biomedical Research, 4056 Basel, Switzerland;
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  • ORCID record for Solange Vidal
Nico Preising
bCore Facility Functional Peptidomics, Ulm University Medical Center, 89081 Ulm, Germany;
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Ludger Ständker
bCore Facility Functional Peptidomics, Ulm University Medical Center, 89081 Ulm, Germany;
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  • ORCID record for Ludger Ständker
Sebastian Wiese
cCore Unit Mass Spectrometry and Proteomics, Ulm University Medical Center, 89081 Ulm, Germany;
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  • ORCID record for Sebastian Wiese
Christina M. Stürzel
aInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;
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Mirja Harms
aInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;
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Rüdiger Gross
aInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;
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  • ORCID record for Rüdiger Gross
Christoph Jung
fInstitute of Electrochemistry, Ulm University, 89081 Ulm, Germany;
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Miriam Kiene
gInfection Biology Unit, German Primate Center–Leibniz Institute for Primate Research, 37077 Göttingen, Germany;
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Timo Jacob
fInstitute of Electrochemistry, Ulm University, 89081 Ulm, Germany;
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  • ORCID record for Timo Jacob
Stefan Pöhlmann
gInfection Biology Unit, German Primate Center–Leibniz Institute for Primate Research, 37077 Göttingen, Germany;
hFaculty of Biology and Psychology, University Göttingen, 37073 Göttingen, Germany;
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Wolf-Georg Forssmann
dPHARIS Biotec GmbH, 30625 Hannover, Germany;
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Jan Münch
aInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;
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  • ORCID record for Jan Münch
Konstantin M. J. Sparrer
aInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;
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Klaus Seuwen
eNovartis Institutes for Biomedical Research, 4056 Basel, Switzerland;
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Beatrice H. Hahn
iDepartment of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076;
jDepartment of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076
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  • For correspondence: bhahn@pennmedicine.upenn.edu frank.kirchhoff@uni-ulm.de
Frank Kirchhoff
aInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;
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  • For correspondence: bhahn@pennmedicine.upenn.edu frank.kirchhoff@uni-ulm.de
  1. Contributed by Beatrice H. Hahn, December 5, 2020 (sent for review November 16, 2020; reviewed by Shibo Jiang and Wesley I. Sundquist)

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Article Information

vol. 118 no. 3 e2023776118
DOI: 
https://doi.org/10.1073/pnas.2023776118
PubMed: 
33431697

Published By: 
National Academy of Sciences
Print ISSN: 
0027-8424
Online ISSN: 
1091-6490
History: 
  • Published in issue January 19, 2021.
  • Published online January 11, 2021.

Copyright & Usage: 
Copyright © 2021 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

Author Information

  1. Manuel Hayna,1,
  2. Andrea Blötza,1,
  3. Armando Rodríguezb,c,d,
  4. Solange Vidale,
  5. Nico Preisingb,
  6. Ludger Ständkerb,
  7. Sebastian Wiesec,
  8. Christina M. Stürzela,
  9. Mirja Harmsa,
  10. Rüdiger Grossa,
  11. Christoph Jungf,
  12. Miriam Kieneg,
  13. Timo Jacobf,
  14. Stefan Pöhlmanng,h,
  15. Wolf-Georg Forssmannd,
  16. Jan Müncha,
  17. Konstantin M. J. Sparrera,
  18. Klaus Seuwene,
  19. Beatrice H. Hahni,j,2, and
  20. Frank Kirchhoffa,2
  1. aInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;
  2. bCore Facility Functional Peptidomics, Ulm University Medical Center, 89081 Ulm, Germany;
  3. cCore Unit Mass Spectrometry and Proteomics, Ulm University Medical Center, 89081 Ulm, Germany;
  4. dPHARIS Biotec GmbH, 30625 Hannover, Germany;
  5. eNovartis Institutes for Biomedical Research, 4056 Basel, Switzerland;
  6. fInstitute of Electrochemistry, Ulm University, 89081 Ulm, Germany;
  7. gInfection Biology Unit, German Primate Center–Leibniz Institute for Primate Research, 37077 Göttingen, Germany;
  8. hFaculty of Biology and Psychology, University Göttingen, 37073 Göttingen, Germany;
  9. iDepartment of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076;
  10. jDepartment of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076
  1. Contributed by Beatrice H. Hahn, December 5, 2020 (sent for review November 16, 2020; reviewed by Shibo Jiang and Wesley I. Sundquist)

Footnotes

  • ↵1M. Hayn and A.B. contributed equally to this work.

  • ↵2To whom correspondence may be addressed. Email: bhahn{at}pennmedicine.upenn.edu or frank.kirchhoff{at}uni-ulm.de.
  • Author contributions: A.B., L.S., S.W., S.P., K.M.J.S., K.S., and F.K. designed research; M. Hayn, A.B., A.R., S.V., N.P., S.W., C.M.S., M. Harms, R.G., C.J., M.K., and T.J. performed research; C.M.S., W.-G.F., J.M., and B.H.H. contributed new reagents/analytic tools; M. Hayn, A.B., A.R., L.S., S.W., C.J., T.J., S.P., K.M.J.S., K.S., and F.K. analyzed data; and B.H.H. and F.K. wrote the paper.

  • Reviewers: S.J., Fudan University; and W.I.S., University of Utah Medical Center.

  • The authors declare no competing interest.

  • This article contains supporting information online at https://www.pnas.org/lookup/suppl/doi:10.1073/pnas.2023776118/-/DCSupplemental.

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Natural cystatin C fragments inhibit GPR15-mediated HIV and SIV infection without interfering with GPR15L signaling
Manuel Hayn, Andrea Blötz, Armando Rodríguez, Solange Vidal, Nico Preising, Ludger Ständker, Sebastian Wiese, Christina M. Stürzel, Mirja Harms, Rüdiger Gross, Christoph Jung, Miriam Kiene, Timo Jacob, Stefan Pöhlmann, Wolf-Georg Forssmann, Jan Münch, Konstantin M. J. Sparrer, Klaus Seuwen, Beatrice H. Hahn, Frank Kirchhoff
Proceedings of the National Academy of Sciences Jan 2021, 118 (3) e2023776118; DOI: 10.1073/pnas.2023776118

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Natural cystatin C fragments inhibit GPR15-mediated HIV and SIV infection without interfering with GPR15L signaling
Manuel Hayn, Andrea Blötz, Armando Rodríguez, Solange Vidal, Nico Preising, Ludger Ständker, Sebastian Wiese, Christina M. Stürzel, Mirja Harms, Rüdiger Gross, Christoph Jung, Miriam Kiene, Timo Jacob, Stefan Pöhlmann, Wolf-Georg Forssmann, Jan Münch, Konstantin M. J. Sparrer, Klaus Seuwen, Beatrice H. Hahn, Frank Kirchhoff
Proceedings of the National Academy of Sciences Jan 2021, 118 (3) e2023776118; DOI: 10.1073/pnas.2023776118
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Proceedings of the National Academy of Sciences: 118 (3)
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