Coronavirus replication–transcription complex: Vital and selective NMPylation of a conserved site in nsp9 by the NiRAN-RdRp subunit

^aInstitute of Medical Virology, Justus Liebig University Giessen, 35392 Giessen, Germany;
^bDepartment of Medical Microbiology, Leiden University Medical Center, Leiden 2300 RC, The Netherlands;
^cDepartment of Biomedical Data Sciences, Leiden University Medical Center, Leiden 2300 RC, The Netherlands;
^dFaculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119991 Moscow, Russia;
^eMass Spectrometry Facility, Department of Chemistry, Philipps University Marburg, 35043 Marburg, Germany

Edited by Peter Sarnow, Stanford University School of Medicine, Stanford, CA, and approved December 25, 2020 (received for review October 25, 2020)

Footnotes

↵¹H.S. and R.M. contributed equally to this work.
↵²Present address: Department of Genetics, University Medical Center Groningen, Groningen 9713 GZ, The Netherlands.
↵³To whom correspondence may be addressed. Email: John.Ziebuhr{at}viro.med.uni-giessen.de.

Author contributions: H.S., R.M., U.L., and J.Z. designed research; H.S., R.M., G.B., K.S., N.K., and U.L. performed research; A.G. and A.E.G. contributed new reagents/analytic tools; H.S., R.M., U.L., and J.Z. analyzed data; and H.S., R.M., A.E.G., U.L., and J.Z. wrote the paper.
The authors declare no competing interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at https://www.pnas.org/lookup/suppl/doi:10.1073/pnas.2022310118/-/DCSupplemental.