Replication and Transcription of Eukaryotic DNA in Esherichia coli

John F. Morrow, Stanley N. Cohen, Annie C. Y. Chang, Herbert W. Boyer, Howard M. Goodman, and Robert B. Helling
PNAS May 1, 1974 71 (5) 1743-1747; https://doi.org/10.1073/pnas.71.5.1743

Abstract

Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.

Back to top
Article Alerts
Email Article
Citation Tools
Request Permissions
Replication and Transcription of Eukaryotic DNA in Esherichia coli
John F. Morrow, Stanley N. Cohen, Annie C. Y. Chang, Herbert W. Boyer, Howard M. Goodman, Robert B. Helling
Proceedings of the National Academy of Sciences May 1974, 71 (5) 1743-1747; DOI: 10.1073/pnas.71.5.1743
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Proceedings of the National Academy of Sciences: 116 (8)
Current Issue

Submit

Jump to section

You May Also be Interested in

Nonmonogamous strawberry poison frog (Oophaga pumilio). Image courtesy of Yusan Yang (University of Pittsburgh, Pittsburgh).
Putative signature of monogamy
A study suggests a putative gene-expression hallmark common to monogamous male vertebrates of some species, namely cichlid fishes, dendrobatid frogs, passeroid songbirds, common voles, and deer mice, and identifies 24 candidate genes potentially associated with monogamy.
Image courtesy of Yusan Yang (University of Pittsburgh, Pittsburgh).
Active lifestyles. Image courtesy of Pixabay/MabelAmber.
Meaningful life tied to healthy aging
Physical and social well-being in old age are linked to self-assessments of life worth, and a spectrum of behavioral, economic, health, and social variables may influence whether aging individuals believe they are leading meaningful lives.
Image courtesy of Pixabay/MabelAmber.