New Research In
Physical Sciences
Social Sciences
Featured Portals
Articles by Topic
Biological Sciences
Featured Portals
Articles by Topic
- Agricultural Sciences
- Anthropology
- Applied Biological Sciences
- Biochemistry
- Biophysics and Computational Biology
- Cell Biology
- Developmental Biology
- Ecology
- Environmental Sciences
- Evolution
- Genetics
- Immunology and Inflammation
- Medical Sciences
- Microbiology
- Neuroscience
- Pharmacology
- Physiology
- Plant Biology
- Population Biology
- Psychological and Cognitive Sciences
- Sustainability Science
- Systems Biology
Light-harvesting chlorophyll a/b-protein: Three-dimensional structure of a reconstituted membrane lattice in negative stain

Abstract
The three-dimensional structure of a negatively stained hexagonal membrane lattice containing the light-harvesting chlorophyll a/b-protein complex and phospholipids has been determined to 30-Å resolution by image reconstruction from electron micrographs. This lattice has p321 symmetry, a lattice constant of 125 Å and a thickness of 75 Å. The monomer is shown to be an elongated molecule about 65 Å long in the dimension perpendicular to the plane of the membrane. It spans the hydrophobic domain of the membrane in an asymmetric fashion, projecting [unk]20 Å from one surface and less from the other. On the basis of this image and available biochemical data, the structure of the complex in the native thylakoid membrane is proposed.
- image reconstruction
- photosynthesis
- integral membrane protein
- two-dimensional crystal
- thylakoid stacking
-
↵† Present address: Medical Research Council Laboratory of Molecular Biology, Hills Rd., Cambridge CB2 2QH, England.