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Research Article

Preparation and properties of nido-carborane-specific monoclonal antibodies for potential use in boron neutron capture therapy for cancer

R H Pak, F J Primus, K J Rickard-Dickson, L L Ng, R R Kane, and M F Hawthorne
  1. Department of Chemistry and Biochemistry, University of California at Los Angeles 90024, USA.

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PNAS July 18, 1995 92 (15) 6986-6990; https://doi.org/10.1073/pnas.92.15.6986
R H Pak
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F J Primus
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K J Rickard-Dickson
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L L Ng
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R R Kane
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M F Hawthorne
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Abstract

As the first step of a research program aimed at developing a bispecific monoclonal antibody system for the delivery of boron-rich molecules to tumor cells for boron neutron capture therapy, monoclonal antibodies (mAbs) were produced against an anionic nido-carborane derivative, 4-[7,8-dicarbadodecahydroundecaborat(-1)-7-yl]butanoic acid. Two IgG subclass mAbs, designated HAW101 and HAW102, were identified that specifically bound the anionic nido-carborane hapten, as well as a variety of other anionic nido-carborane cage derivatives. By using surface plasmon resonance technology, the affinity constants of HAW101 and HAW102 were determined to be 1.9 x 10(9) and 6.8 x 10(8) M-1, respectively. A diverse array of 7-substituted and 7,8-disubstituted anionic nido-carborane derivatives reacted with the mAb HAW101 in competition ELISA, whereas anionic closo-polyhedral boranes showed negligible binding, suggesting a role for the open nido-carborane cage structure. These results suggest that mAbs such as HAW101, which bind anionic nido-carboranes, are useful in the development of bispecific mAbs for specific targeting and enhanced boron delivery to tumor sites.

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Preparation and properties of nido-carborane-specific monoclonal antibodies for potential use in boron neutron capture therapy for cancer
R H Pak, F J Primus, K J Rickard-Dickson, L L Ng, R R Kane, M F Hawthorne
Proceedings of the National Academy of Sciences Jul 1995, 92 (15) 6986-6990; DOI: 10.1073/pnas.92.15.6986

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Preparation and properties of nido-carborane-specific monoclonal antibodies for potential use in boron neutron capture therapy for cancer
R H Pak, F J Primus, K J Rickard-Dickson, L L Ng, R R Kane, M F Hawthorne
Proceedings of the National Academy of Sciences Jul 1995, 92 (15) 6986-6990; DOI: 10.1073/pnas.92.15.6986
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