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Research Article

E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle

C Sardet, M Vidal, D Cobrinik, Y Geng, C Onufryk, A Chen, and R A Weinberg
PNAS March 14, 1995 92 (6) 2403-2407; https://doi.org/10.1073/pnas.92.6.2403
C Sardet
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M Vidal
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D Cobrinik
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Y Geng
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C Onufryk
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A Chen
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R A Weinberg
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Abstract

The E2F transcription factors play a role in regulating the expression of genes required for cell proliferation. Their activity appears to be regulated by association with the retinoblastoma protein (pRb) and the pRb-related proteins p107 and p130. In vivo, pRb is found in complex with a subset of E2F components--namely, E2F-1, E2F-2, and E2F-3. Here we describe the characterization of cDNAs encoding two unusual E2Fs, E2F-4 and E2F-5, each identified by the ability of their gene product to interact with p130 in a yeast two-hybrid system. E2F-4 and -5 share common sequences with E2F-1, E2F-2, and E2F-3 and, like these other E2Fs, the ability to heterodimerize with DP-1, thereby acquiring the ability to bind an E2F DNA recognition sequence with high affinity. However, in contrast to E2F-1, E2F-4 and E2F-5 fail to bind pRb in a two-hybrid assay. Moreover, they show a unique pattern of expression in synchronized human keratinocytes: E2F-4 and E2F-5 mRNA expression is maximal in mid-G1 phase before E2F-1 expression is detectable. These findings suggest that E2F-4 and E2F-5 may contribute to the regulation of early G1 events including the G0/G1 transition.

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E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle
C Sardet, M Vidal, D Cobrinik, Y Geng, C Onufryk, A Chen, R A Weinberg
Proceedings of the National Academy of Sciences Mar 1995, 92 (6) 2403-2407; DOI: 10.1073/pnas.92.6.2403

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E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle
C Sardet, M Vidal, D Cobrinik, Y Geng, C Onufryk, A Chen, R A Weinberg
Proceedings of the National Academy of Sciences Mar 1995, 92 (6) 2403-2407; DOI: 10.1073/pnas.92.6.2403
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