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Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA

Ya-Bin Lu, Pillarisetty V. A. L. Ratnakar, Bidyut K. Mohanty, and Deepak Bastia
PNAS November 12, 1996 93 (23) 12902-12907; https://doi.org/10.1073/pnas.93.23.12902
Ya-Bin Lu
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Pillarisetty V. A. L. Ratnakar
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Bidyut K. Mohanty
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Deepak Bastia
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The primase DnaG of Escherichia coli requires the participation of the replicative helicase DnaB for optimal synthesis of primer RNA for lagging strand replication. However, previous studies had not determined whether the activation of the primase or its loading on the template was accomplished by a helicase-mediated structural alteration of the single-stranded DNA or by a direct physical interaction between the DnaB and the DnaG proteins. In this paper we present evidence supporting direct interaction between the two proteins. We have mapped the surfaces of interaction on both DnaG and DnaB and show further that mutations that reduce the physical interaction also cause a significant reduction in primer synthesis. Thus, the physical interaction reported here appears to be physiologically significant.

  • protein–protein interaction
  • lagging strand replication

Footnotes

    • ↵ To whom reprint requests should be addressed.

    • Wolfgang K. Joklik, Duke University Medical Center, Durham, NC

    • Abbreviations: wt, wild type; GST, glutathione S-transferase, tr.BC3, truncated BC3; ssDNA, single-stranded DNA.

    • Received July 2, 1996.
    • Accepted September 3, 1996.
    • Copyright © 1996, The National Academy of Sciences of the USA
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    Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA
    Ya-Bin Lu, Pillarisetty V. A. L. Ratnakar, Bidyut K. Mohanty, Deepak Bastia
    Proceedings of the National Academy of Sciences Nov 1996, 93 (23) 12902-12907; DOI: 10.1073/pnas.93.23.12902

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    Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA
    Ya-Bin Lu, Pillarisetty V. A. L. Ratnakar, Bidyut K. Mohanty, Deepak Bastia
    Proceedings of the National Academy of Sciences Nov 1996, 93 (23) 12902-12907; DOI: 10.1073/pnas.93.23.12902
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