Skip to main content
  • Submit
  • About
    • Editorial Board
    • PNAS Staff
    • FAQ
    • Accessibility Statement
    • Rights and Permissions
    • Site Map
  • Contact
  • Journal Club
  • Subscribe
    • Subscription Rates
    • Subscriptions FAQ
    • Open Access
    • Recommend PNAS to Your Librarian
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Current
    • Special Feature Articles - Most Recent
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • List of Issues
  • Front Matter
  • News
    • For the Press
    • This Week In PNAS
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Editorial and Journal Policies
    • Submission Procedures
    • Fees and Licenses
  • Submit
  • About
    • Editorial Board
    • PNAS Staff
    • FAQ
    • Accessibility Statement
    • Rights and Permissions
    • Site Map
  • Contact
  • Journal Club
  • Subscribe
    • Subscription Rates
    • Subscriptions FAQ
    • Open Access
    • Recommend PNAS to Your Librarian

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Home
Home

Advanced Search

  • Home
  • Articles
    • Current
    • Special Feature Articles - Most Recent
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • List of Issues
  • Front Matter
  • News
    • For the Press
    • This Week In PNAS
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Editorial and Journal Policies
    • Submission Procedures
    • Fees and Licenses

New Research In

Physical Sciences

Featured Portals

  • Physics
  • Chemistry
  • Sustainability Science

Articles by Topic

  • Applied Mathematics
  • Applied Physical Sciences
  • Astronomy
  • Computer Sciences
  • Earth, Atmospheric, and Planetary Sciences
  • Engineering
  • Environmental Sciences
  • Mathematics
  • Statistics

Social Sciences

Featured Portals

  • Anthropology
  • Sustainability Science

Articles by Topic

  • Economic Sciences
  • Environmental Sciences
  • Political Sciences
  • Psychological and Cognitive Sciences
  • Social Sciences

Biological Sciences

Featured Portals

  • Sustainability Science

Articles by Topic

  • Agricultural Sciences
  • Anthropology
  • Applied Biological Sciences
  • Biochemistry
  • Biophysics and Computational Biology
  • Cell Biology
  • Developmental Biology
  • Ecology
  • Environmental Sciences
  • Evolution
  • Genetics
  • Immunology and Inflammation
  • Medical Sciences
  • Microbiology
  • Neuroscience
  • Pharmacology
  • Physiology
  • Plant Biology
  • Population Biology
  • Psychological and Cognitive Sciences
  • Sustainability Science
  • Systems Biology
Research Article

Reduced stress defense in heme oxygenase 1-deficient cells

Kenneth D. Poss and Susumu Tonegawa
PNAS September 30, 1997 94 (20) 10925-10930; https://doi.org/10.1073/pnas.94.20.10925
Kenneth D. Poss
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susumu Tonegawa
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  1. Contributed by Susumu Tonegawa

  • Article
  • Figures & SI
  • Info & Metrics
  • PDF
Loading

Abstract

Stressed mammalian cells up-regulate heme oxygenase 1 (Hmox1; EC 1.14.99.3), which catabolizes heme to biliverdin, carbon monoxide, and free iron. To assess the potential role of Hmox1 in cellular antioxidant defense, we analyzed the responses of cells from mice lacking functional Hmox1 to oxidative challenges. Cultured Hmox1−/− embryonic fibroblasts demonstrated high oxygen free radical production when exposed to hemin, hydrogen peroxide, paraquat, or cadmium chloride, and they were hypersensitive to cytotoxicity caused by hemin and hydrogen peroxide. Furthermore, young adult Hmox1−/− mice were vulnerable to mortality and hepatic necrosis when challenged with endotoxin. Our in vitro and in vivo results provide genetic evidence that up-regulation of Hmox1 serves as an adaptive mechanism to protect cells from oxidative damage during stress.

Footnotes

    • ↵* To whom reprint requests should be addressed at: Center for Cancer Research, E17-346, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139. e-mail: kdposs{at}mit.edu.

  • ABBREVIATIONS

    Hmox,
    heme oxygenase;
    E12.5,
    embryonic day 12.5;
    MEFs,
    murine embryonic fibroblasts;
    LPS,
    lipopolysaccharide
    • Accepted July 16, 1997.
    • Copyright © 1997, The National Academy of Sciences of the USA
    View Full Text
    PreviousNext
    Back to top
    Article Alerts
    Email Article

    Thank you for your interest in spreading the word on PNAS.

    NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

    Enter multiple addresses on separate lines or separate them with commas.
    Reduced stress defense in heme oxygenase 1-deficient cells
    (Your Name) has sent you a message from PNAS
    (Your Name) thought you would like to see the PNAS web site.
    CAPTCHA
    This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
    Citation Tools
    Reduced stress defense in heme oxygenase 1-deficient cells
    Kenneth D. Poss, Susumu Tonegawa
    Proceedings of the National Academy of Sciences Sep 1997, 94 (20) 10925-10930; DOI: 10.1073/pnas.94.20.10925

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
    Request Permissions
    Share
    Reduced stress defense in heme oxygenase 1-deficient cells
    Kenneth D. Poss, Susumu Tonegawa
    Proceedings of the National Academy of Sciences Sep 1997, 94 (20) 10925-10930; DOI: 10.1073/pnas.94.20.10925
    Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
    • Tweet Widget
    • Facebook Like
    • Mendeley logo Mendeley
    Table of Contents

    Submit

    Sign up for Article Alerts

    Jump to section

    • Article
      • Abstract
      • MATERIALS AND METHODS
      • RESULTS
      • DISCUSSION
      • Acknowledgments
      • Footnotes
      • ABBREVIATIONS
      • References
    • Figures & SI
    • Info & Metrics
    • PDF

    You May Also be Interested in

    Surgeons hands during surgery
    Inner Workings: Advances in infectious disease treatment promise to expand the pool of donor organs
    Despite myriad challenges, clinicians see room for progress.
    Image credit: Shutterstock/David Tadevosian.
    Setting sun over a sun-baked dirt landscape
    Core Concept: Popular integrated assessment climate policy models have key caveats
    Better explicating the strengths and shortcomings of these models will help refine projections and improve transparency in the years ahead.
    Image credit: Witsawat.S.
    Double helix
    Journal Club: Noncoding DNA shown to underlie function, cause limb malformations
    Using CRISPR, researchers showed that a region some used to label “junk DNA” has a major role in a rare genetic disorder.
    Image credit: Nathan Devery.
    Steamboat Geyser eruption.
    Eruption of Steamboat Geyser
    Mara Reed and Michael Manga explore why Yellowstone's Steamboat Geyser resumed erupting in 2018.
    Listen
    Past PodcastsSubscribe
    Multi-color molecular model
    Enzymatic breakdown of PET plastic
    A study demonstrates how two enzymes—MHETase and PETase—work synergistically to depolymerize the plastic pollutant PET.
    Image credit: Aaron McGeehan (artist).

    Similar Articles

    Site Logo
    Powered by HighWire
    • Submit Manuscript
    • Twitter
    • Facebook
    • RSS Feeds
    • Email Alerts

    Articles

    • Current Issue
    • Special Feature Articles – Most Recent
    • List of Issues

    PNAS Portals

    • Anthropology
    • Chemistry
    • Classics
    • Front Matter
    • Physics
    • Sustainability Science
    • Teaching Resources

    Information

    • Authors
    • Editorial Board
    • Reviewers
    • Librarians
    • Press
    • Site Map
    • PNAS Updates

    Feedback    Privacy/Legal

    Copyright © 2021 National Academy of Sciences. Online ISSN 1091-6490