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Research Article

Rapid decline of fitness in panmictic populations of Drosophila melanogaster maintained under relaxed natural selection

Svetlana A. Shabalina, Lev Yu. Yampolsky, and Alexey S. Kondrashov
PNAS November 25, 1997 94 (24) 13034-13039; https://doi.org/10.1073/pnas.94.24.13034
Svetlana A. Shabalina
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Lev Yu. Yampolsky
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Alexey S. Kondrashov
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  1. Edited by John Maynard Smith, University of Sussex, Brighton, United Kingdom, and approved September 12, 1997 (received for review July 21, 1997)

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    Figure 1

    Egg-to-imago mortalities in the experimental populations (MCN-1, solid lines; MCN-2, broken lines) and their linear regressions on generation number t (0.1506 + 0.0012t in MCN-1 and 0.1829 + 0.0021t in MCN-2). The data for generation 15 are absent because back-up vials were used. The infecundity rate was ≈1% in MCN-1 and ≈2% in MCN-2.

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    Figure 2

    Distributions of fecundities of the outbred and inbred frozen control females, outbred and inbred MCN-1 females, and females originated in the frozen control × MCN-1 cross in the third measurement of fitness-related traits.

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    Figure 3

    Means, SEs, and linear regressions on the generation number of the differences between the competitive abilities of MCN-1 (solid lines) or MCN-2 (broken lines) and the average competitive ability of the two controls.

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    Table 1

    Fecundity

    GenerationFrozen controlCold controlMCN-1MCN-2
    10105.04  ± 1.68 (4)103.90  ± 1.51 (6)96.68  ± 2.28 (7)92.99  ± 1.88 (4)
    2097.53  ± 1.42 (2)88.62  ± 1.99 (1)91.06  ± 1.96 (4)90.47  ± 1.52 (3)
    30105.30  ± 1.83 (7)108.15  ± 1.94 (4)99.69  ± 1.93 (6)93.68  ± 2.49 (3)
    Regression coefficient0.00049−0.00049 −0.00225 −0.00376
    ±0.00044±0.00050 ±0.00056 ±0.00054
    • Fecundities deviating from the mean by more than 3 SD (calculated for all nonsterile females) were discounted. The total numbers of discounted females are given in parentheses. 

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    Table 2

    Competitive ability

    GenerationFrozen controlCold controlMCN1MCN2
    Dark eye reference line, Density 6:6
     10−0.332  ±  0.205−0.276  ±  0.115−0.480  ±  0.109−0.689  ±  0.112
     200.618  ±  0.1570.151  ±  0.2660.581  ±  0.213−0.209  ±  0.114
     30−0.926  ±  0.293−0.611  ±  0.175−1.468  ±  0.285−1.474  ±  0.163
     Regression coefficient0.0002−0.0002 −0.0128 −0.0264
    ±0.0060±0.0052 ±0.0059 ±0.0035
    Dark eye reference line, Density 12:12
     10−0.083  ±  0.152−0.165  ±  0.159−0.854  ±  0.189−0.561  ±  0.232
     200.654  ±  0.1530.414  ±  0.3120.107  ±  0.155−0.337  ±  0.114
     30−1.005  ±  0.177−1.258  ±  0.189−1.736  ±  0.283−2.058  ±  0.190
     Regression coefficient0.0044−0.0048 −0.0248 −0.0359
    ±0.0042±0.0055 ±0.0059 ±0.0051
    Ebony reference line, Density 6:6
     100.269  ±  0.1610.248  ±  0.1670.117  ±  0.1420.264  ±  0.236
     200.808  ±  0.189−0.041  ±  0.1820.627  ±  0.1540.146  ±  0.276
     30−0.268  ±  0.153−0.192  ±  0.121−1.106  ±  0.326−1.307  ±  0.216
     Regression coefficient0.0049−0.0050 −0.0165 −0.0272
    ±0.0046±0.0043 ±0.0063 ±0.0065
    Ebony reference line, Density 12:12
     101.127  ±  0.1610.962  ±  0.1380.399  ±  0.1940.903  ±  0.197
     200.523  ±  0.2490.203  ±  0.2160.263  ±  0.264
     30−1.673  ±  0.255−1.030  ±  0.266−2.108  ±  0.249−1.513  ±  0.282
     Regression coefficient−0.00370.0048 −0.0293 −0.0057
    ±0.0061±0.0057 ±0.0073 ±0.0066
    • The missing entry is due to an experimental error. 

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    Table 3

    Mean and variance components of competitive ability in small vials

    Frozen controlCold controlMCN-1
    Mean0.713  ±  0.0480.632  ±  0.0320.563  ±  0.032
    Sire0.026  ±  0.0100.025  ±  0.0210.018  ±  0.013
    Dam0.040  ±  0.0340.059  ±  0.0180.058  ±  0.018
    Residual0.063  ±  0.0280.057  ±  0.0140.067  ±  0.015
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    Table 4

    Motility

    GenerationFrozen controlCold controlMCN-1MCN-2
    102.80  ±  0.0652.62  ±  0.0892.59  ±  0.0852.44  ±  0.080
    202.58  ±  0.0882.55  ±  0.1162.51  ±  0.0842.44  ±  0.111
    302.70  ±  0.1312.72  ±  0.0972.61  ±  0.0822.70  ±  0.106
    Regression coefficient0.00067−0.00007 −0.00187 −0.00291
    ±0.00078±0.00075 ±0.00069 ±0.00075
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Rapid decline of fitness in panmictic populations of Drosophila melanogaster maintained under relaxed natural selection
Svetlana A. Shabalina, Lev Yu. Yampolsky, Alexey S. Kondrashov
Proceedings of the National Academy of Sciences Nov 1997, 94 (24) 13034-13039; DOI: 10.1073/pnas.94.24.13034

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Rapid decline of fitness in panmictic populations of Drosophila melanogaster maintained under relaxed natural selection
Svetlana A. Shabalina, Lev Yu. Yampolsky, Alexey S. Kondrashov
Proceedings of the National Academy of Sciences Nov 1997, 94 (24) 13034-13039; DOI: 10.1073/pnas.94.24.13034
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