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Research Article

Normal retina releases a diffusible factor stimulating cone survival in the retinal degeneration mouse

Saddek Mohand-Said, Alain Deudon-Combe, David Hicks, Manuel Simonutti, Valérie Forster, Anne-Claire Fintz, Thierry Léveillard, Henri Dreyfus, and José-Alain Sahel
  1. Laboratoire de Physiopathologie Rétinienne, Université Louis Pasteur, Clinique Ophtalmologique, Centre Hospitalier et Universitaire, BP 426, 1, Place de l’Hôpital, 67091 Strasbourg Cedex, France

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PNAS July 7, 1998 95 (14) 8357-8362; https://doi.org/10.1073/pnas.95.14.8357
Saddek Mohand-Said
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Alain Deudon-Combe
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David Hicks
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Manuel Simonutti
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Valérie Forster
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Anne-Claire Fintz
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Thierry Léveillard
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Henri Dreyfus
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José-Alain Sahel
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  1. Communicated by John E. Dowling, Harvard University, Cambridge, MA (received for review July 21, 1997)

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Abstract

The role of cellular interactions in the mechanism of secondary cone photoreceptor degeneration in inherited retinal degenerations in which the mutation specifically affects rod photoreceptors was studied. We developed an organ culture model of whole retinas from 5-week-old mice carrying the retinal degeneration mutation, which at this age contain few remaining rods and numerous surviving cones cocultured with primary cultures of mixed cells from postnatal day 8 normal-sighted mice (C57BL/6) retinas or retinal explants from normal (C57BL/6) or dystrophic (C3H/He) 5-week-old mice. After 7 days, the numbers of residual cone photoreceptors were quantified after specific peanut lectin or anti-arrestin antibody labeling by using an unbiased stereological approach. Examination of organ cultured retinas revealed significantly greater numbers of surviving cones (15–20%) if cultured in the presence of retinas containing normal rods as compared with controls or cocultures with rod-deprived retinas. These data indicate the existence of a diffusible trophic factor released from retinas containing rod cells and acting on retinas in which only cones are present. Because cones are responsible for high acuity and color vision, such data could have important implications not only for eventual therapeutic approaches to human retinal degenerations but also to define interactions between retinal photoreceptor types.

Footnotes

    • ↵* S.M.-S. and A.D.-C. should be considered equal first authors.

    • ↵† To whom reprint requests should be addressed. e-mail: sahel{at}neurochem.u-strasbg.fr.

  • ABBREVIATIONS

    rd,
    retinal degeneration;
    RP,
    retinitis pigmentosa;
    PNA peanut agglutinin.,
    • Received July 21, 1997.
    • Accepted May 6, 1998.
    • Copyright © 1998, The National Academy of Sciences
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    Normal retina releases a diffusible factor stimulating cone survival in the retinal degeneration mouse
    Saddek Mohand-Said, Alain Deudon-Combe, David Hicks, Manuel Simonutti, Valérie Forster, Anne-Claire Fintz, Thierry Léveillard, Henri Dreyfus, José-Alain Sahel
    Proceedings of the National Academy of Sciences Jul 1998, 95 (14) 8357-8362; DOI: 10.1073/pnas.95.14.8357

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    Normal retina releases a diffusible factor stimulating cone survival in the retinal degeneration mouse
    Saddek Mohand-Said, Alain Deudon-Combe, David Hicks, Manuel Simonutti, Valérie Forster, Anne-Claire Fintz, Thierry Léveillard, Henri Dreyfus, José-Alain Sahel
    Proceedings of the National Academy of Sciences Jul 1998, 95 (14) 8357-8362; DOI: 10.1073/pnas.95.14.8357
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