New Research In
Physical Sciences
Social Sciences
Featured Portals
Articles by Topic
Biological Sciences
Featured Portals
Articles by Topic
 Agricultural Sciences
 Anthropology
 Applied Biological Sciences
 Biochemistry
 Biophysics and Computational Biology
 Cell Biology
 Developmental Biology
 Ecology
 Environmental Sciences
 Evolution
 Genetics
 Immunology and Inflammation
 Medical Sciences
 Microbiology
 Neuroscience
 Pharmacology
 Physiology
 Plant Biology
 Population Biology
 Psychological and Cognitive Sciences
 Sustainability Science
 Systems Biology
Maximum likelihood estimation of a migration matrix and effective population sizes in n subpopulations by using a coalescent approach

Contributed by Joseph Felsenstein
Abstract
A maximum likelihood estimator based on the coalescent for unequal migration rates and different subpopulation sizes is developed. The method uses a Markov chain Monte Carlo approach to investigate possible genealogies with branch lengths and with migration events. Properties of the new method are shown by using simulated data from a fourpopulation nisland model and a source–sink population model. Our estimation method as coded in migrate is tested against genetree; both programs deliver a very similar likelihood surface. The algorithm converges to the estimates fairly quickly, even when the Markov chain is started from unfavorable parameters. The method was used to estimate gene flow in the Nile valley by using mtDNA data from three human populations.
Estimation of migration rates from genetic data has a long history. As soon as the first analyses of population samples by using enzyme electrophoresis were available, migration rates estimated from Wright's F_{ST} (1) were used to infer patterns of gene flow. With the advent of other types of genetic data, such as restriction fragment length polymorphism data, DNA sequences, and microsatellite loci, migration rates have been routinely estimated by using modified versions of F_{ST} (2–6). Translation of these F_{ST} equivalents into migration rate estimates most often assumes that all subpopulations have the same size, or that there are infinitely many subpopulations, and that the migration rates are all symmetric. If the true migration pattern has been asymmetric, or the subpopulation sizes are unequal, F_{ST}based methods will deliver wrong estimates (7).
Recently, it became possible to estimate migration rates and population sizes without the assumption that subpopulation sizes are all equal (8) or that the migration rates between the subpopulations are symmetric (9–12).
We describe here an extension of our twopopulation method (10) that calculates maximum likelihood estimates of migration rates and subpopulation sizes by using coalescence theory (13, 14). Our method allows us to analyze more than two subpopulations, to specify arbitrary migration scenarios, and to test a hierarchy of different migration scenarios. Success of estimating migration pattern is assessed with simulated sequence data in an nisland population model and a source–sink population scenario. Convergence to the correct result is investigated through simulation. The performance of our method, implemented in the program migrate, is compared with that of genetree (11). Finally, we analyze a human mtDNA hypervariable region I data set from three populations in the Nile valley, a total of 225 individuals.
Materials and Methods
Model.
We infer the population parameters by using a maximum likelihood approach based on coalescence theory (13, 14). This likelihood is the probability of the data given the parameters 𝒫 1 where Prob(𝒢𝒫) is the probability of a genealogy 𝒢 given the population parameters 𝒫, such as population size (15), exponential population growth rate (16), migration rates (10), and recombination rate (17, 18).
Prob(𝒟𝒢) is the likelihood of the data given the genealogy; this quantity is widely used in phylogenetic inference (19, 20). In this paper, we focus on estimation of migration rates and population sizes while assuming a molecular clock at each locus. For a system with n populations, we use the following set of parameters: 2 where ℳ_{ji} is m_{ji}/μ, where m_{ji} is the immigration rate from population j into i, and μ is the mutation rate per generation. For sequence data, μ is the mutation rate per site and for allelic data, such as allozyme or microsatellite markers, and μ is the mutation rate per locus. Θ_{i} is 4Nμ, where N is the effective population size of population i in a Wright–Fisher population model. Sometimes we use γ_{ji}, which is Θ_{i}ℳ_{ji} = 4Nm_{ji}.
Kingman's coalescent can be extended to include migration (21). Instead of just one type of event, the coalescence of lineages, we need to record n^{2} different events: coalescences in different subpopulations and migration events that switch lineages from one population to another. This migration–coalescence prior is a product over all time intervals T on the genealogy (21). Going backwards in time and using a time scale in which the units of time is the ratio of the generation time and the expectation of the mutation rate, we have 3 The exponential term is the probability that in the jth time interval with length u_{j} neither a migration nor a coalescent event happens; u_{j} is scaled by generations and mutation rate. The remaining term is the point probability density of the actual event that happens. The events in genealogy 𝒢 are either migrations from subpopulation w_{j} to v_{j} or coalescences in subpopulation v_{j}. The indicator variable δ_{j} is 1 when the event at the bottom of interval j is a migration event and is 0 otherwise, and k_{ji} is the number of lineages in subpopulation i during time interval j. No modification of the genealogy likelihood Prob(𝒟𝒢) is necessary to accommodate migration events, as they occur independently of mutation. The modified coalescent probability (3) and the genealogy likelihood are rather easy to calculate.
Unfortunately, the sum of the probabilities of all possible genealogies with different topologies and branch lengths cannot be calculated because there are infinitely many of them and no analytical solution for the integral over branch lengths or topologies is available. But it can be approximated by using the Metropolis–Hastings Markov chain Monte Carlo approach (22, 23). This approach (which we denote MH) concentrates the sampling of genealogies 𝒢 in those regions of the genealogy space that contribute most to the final likelihood. With MH importance sampling, we compute instead of the likelihood L(𝒫) the ratio L(𝒫)/L(𝒫_{0}), where 𝒫_{0} are the parameters that were used to sample the genealogies. This is 4 where g is the number of sampled genealogies. Our derivation of formula 4 from formula 1 was described earlier (10) and is essentially a standard MH scheme (24). The denominator L(𝒫_{0}) is unknown, so that we cannot estimate the absolute likelihood, but this likelihood ratio is proportional to the absolute likelihood function. The parameter estimates obtained by maximizing this approximate likelihood ratio will approach the maximum likelihood estimates as the number of sampled genealogies g becomes infinite (22, 23). We find that very good estimates are achieved with a moderate number of genealogies (10, 15–17).
Our MH approach needs a set of starting parameters 𝒫_{0} and an initial genealogy. These starting parameters can often be found by using a simpler method, such as methods based on F_{ST} (25). The first genealogy is created by using a UPGMA (Unweighted Pair Group Method using arithmetic averages) method (see ref. 19) to construct the topology, and by using Sankoff's parsimony method (26) to reconstruct the minimal number of migrations on that topology. The branch lengths of this initial genealogy are drawn randomly from a coalescent density for that topology given 𝒫_{0}. Our MH method (10) moves through genealogy space by making small rearrangements of branches of the current genealogy. A new genealogy is accepted with probability equal to the ratio of the likelihood of the old genealogy and the likelihood of the new [(for details, see ref. 10) r = min(1, Prob(DG_{new})/Prob(DG_{old})].
With a random or otherwise inappropriate starting genealogy and an inappropriate 𝒫_{0}, the program can spend much time in regions with highly improbable genealogies. To overcome these starting conditions, we use an adaptive scheme that samples 10 short chains, in each of which several thousand genealogies are sampled, followed by two or three long chains, each of which samples many tens or hundreds of thousands of genealogies. After each chain, we reestimate the parameters 𝒫 by maximizing the likelihood ratio (4). These 𝒫 are taken as the 𝒫_{0} of the next chain. The last long chain is used for the final estimates of 𝒫; the earlier chains are used only to obtain good starting parameters.
One would like to know not only the maximum likelihood values of the population parameters, but also confidence intervals for these parameters. Approximate confidence intervals can be generated in a maximum likelihood framework by using either the curvature of the likelihood at its maximum or profile likelihoods (27). The latter are more appropriate for our purposes, as curvaturebased estimates can be unreliable with many parameters unless there are many loci. For an approximative confidence interval for a single parameter, we compare twice the logarithm of the ratio of its profile likelihoods to the quantiles of the χ^{2}distribution with one degree of freedom (27) for the desired level of confidence. If a researcher needs multiparameter confidence intervals, she would need to use a Bonferroni correction or a likelihood ratio test with the correct number of degrees of freedom. The latter method is implemented in migrate. This likelihoodratiobased approach may be inappropriate for data that in theory cannot be extended, such as mtDNA, because the χ^{2} approximation becomes exact only as we add a large number of loci.
Results
Simulation Study.
Data sets were created by using an approach first described by Hudson (28). For some given set of true parameters Θ_{i} and 4N^{(i)}m_{ji}, a coalescent genealogy is created. This genealogy is then used to evolve sites according to the Kimura twoparameter substitution model (29), starting at the root of that genealogy. The sites resulting at the tips are taken as the data. We used 500 sites for each locus in all simulations, except for the comparison of our own results with those from genetree (11).
nisland model.
We simulated a 4island model and were generating 100 10locus data sets with 25 individuals sampled from each of 4 subpopulations (Fig. 1).
The values for the Θ_{i} were taken to be equal and set to 0.01, a value that is moderately close to the estimate of Θ = 0.039 from mtDNA control region domain I sequences from the NuuChahNulth people (15). The migration parameters 4Nm were all set to 1.0. Data sets were analyzed twice, once under the assumption that this is a symmetric nisland model with two parameters Θ and 4Nm, which are the same in all populations, and once under the assumption that we have n^{2} parameters, n different Θ_{i} and n(n − 1) different 4N_{i}m_{ji}.
The averages for the two parameters of the nisland model were Θ̄ = 0.00999 with an SE of 0.00007 and = 0.96327 with an SE of 0.01351. Averages of the limits of the oneparameter 95% profile confidence intervals were (0.00888, 0.01089) and (0.86656, 1.12981), respectively. The estimates for the full migration matrix model with all 16 parameters is shown in Table 1. The estimates for the Θ_{i} are surprisingly precise given the parameterrich model, but most of the 4N_{i}m_{ji} are lower than the true value parameter, although the averages of the individual 95% profile confidence intervals include the true parameter values.
Source–sink model.
The full model with n population sizes and n(n − 1) migration rates is able to detect asymmetric gene flow and differences in population sizes. But for some analyses, this freedom is undesirable because the researcher already has some idea of the pattern of gene flow or the population sizes and may want to fix population sizes, force migration rates to be symmetric, use equal migration rates, or set some migration rates to zero. This goal can be achieved in migrate by using a migration connection matrix (http://evolution.genetics.washington.edu/lamarc/migratedoc/migratedoc.html), such as the one shown in Fig. 2.
One hundred simulated 10locus data sets from the populations shown in Fig. 2 were analyzed by using the full set of 16 parameters, and 50 data sets were analyzed by using only the 7 parameters implied by the migrationconnection matrix.
Some of the simulations in Table 2 do not recover the true values of the migration parameters very well: all parameters with true parameter values of 0.0 are overestimated. Most disturbing are the values for migrations from population 2 to 1, from 3 to 2, and from 4 to 1. But this fact is not surprising, as all parameters are bounded by zero but have no upper limit. Our estimates must deliver a value greater than zero, so that the result must be an upwards bias. In addition, if we do not know the directionality of gene flow, finding the same haplotype in two or more populations will force the program to estimate at least a small migration rate in the wrong direction. Only a few mutations will arise in the small population and be visible in the sample, and only those unique mutations would contribute to inferring that that gene flow from the small population to the big population is very small. Thus, we would need many loci to establish this directional pattern.
If we know the migration model and need to estimate only 7 instead of 16 parameters, the maximum likelihood estimates are almost identical to those for the parameterrich model shown in Table 2, but the profile confidence intervals are slightly smaller: the coefficients of variation (CV) of the parameters are about 25% smaller than with the full model. For example, with 7 parameters, the CV for γ_{12} is 0.299, whereas for the full model, the CV for γ_{12} is 0.392.
Convergence of Our Metropolis–Hastings Sampling Method.
Our Metropolis–Hastings Markov chain algorithm is irreducible, as it can reach any possible genealogy from any other. However, it may take a very long time until the proper regions of the parameter space are found, because the algorithm is sensitive to the start parameters. This problem is specific not to our algorithm but to any MH algorithms that draw correlated samples. There is no simple criterion to judge when the program has converged to the best possible answer. Several convergence measures have been suggested, but there is no guarantee that convergence occurs in a given run (30). We have used a simple graphical method to explore convergence of the twoparameter model (Fig. 3). A singlelocus data set of DNA sequences with 500 bp with 100 individuals sampled from a symmetric model with 4 populations was generated with Θ = 0.01 and ℳ = 100. We ran four cases each with starting parameters (Θ_{0}, ℳ_{0}) equal to (0.0001, 2), (0.001, 200), (0.1, 200), and (0.1, 2) (see Fig. 3). Each run had 10 short chains, each with a total of 20,000 genealogies, and 3 long chains each with a total of 110,000 genealogies. The first 10,000 genealogies in each chain were discarded. At the end of each chain, the parameters were estimated and recorded, and the next chain was then started with these new parameters. These four cases were then compared with a very long run (five times longer) that started from estimates Θ_{0} and ℳ_{0}, which were based on F_{ST} estimates.
The starting points 𝒫_{0} chosen for this convergence study are fairly far from the true parameter values (see ref. 31), but the adaptive improvement of the 𝒫_{0} moves gradually toward parameters of highest likelihood for this data set, as can be seen in Fig. 3: in A, B, D, and F, the trajectories are moving toward values close to the true parameters, namely toward the maximum likelihood estimates for this specific data set, 𝒫_{data}. In Fig. 3C, the trajectory first moves toward high ℳ values (256.4, outside of the shown frame) while staying at low Θ (0.0066) and then returns toward 𝒫_{data}.
Comparison with genetree.
genetree (11) can use only sequence data that evolve according to an infinitelymanysites model. To approximate this model, we simulated data according to the Kimura twoparameter model, but when more than one mutation occurred on the genealogy for a given site, we split the site into two or more new sites, so that each of these new sites would have mutated only once or not at all. We simulated sequence data for 100 loci with 500 bp each that evolved according to this infinitesites model with 2 populations with 2 sampled individuals each. The subpopulations had the same size (Θ_{i} = 0.002) and had a symmetric migration model with rate 4Nm = 0.1. This data set was analyzed with genetree after removal of the invariant sites and also analyzed with migrate for the full set of sites. We chose to evaluate a data set with few individuals and these parameters so that we can compare the outcomes of both programs independent of their ability to search the genealogy space. With genetree, we sampled 1,000 genealogies per locus and used the true parameters to run the sampling process. Our true parameters defined the driving parameters for genetree to be θ_{0} = 4, and 4Nm_{0} = 0.2. The θ_{0} and 4Nm_{0} used in genetree are computed from the mutation rate per locus, and N is the size of the whole population. migrate was run at its default values, except for the following settings: the starting 𝒫_{0} were set to the true values, and the lengths of sampling were set to 10 short chains each of 400 genealogies, of which the first 100 were discarded and then every third genealogy used for the parameter estimation, and then two long chains with 1,600 genealogies each, of which the first 100 were discarded and then every third one was used for parameter estimation. This was a total of 7,200 genealogies visited per locus.
With a large number of loci, one expects that the results should converge to the values used to generate the data. With 100 loci and only 4 sampled individuals, there is much uncertainty about the parameters, but both programs include the true parameter values in their 50% confidence regions (Fig. 4).
migrate spent 45 min for the whole 100locus data set on a computer with a 166MHz Pentium processor, running linux. It was sampling ≈271 genealogies with 4 tips per second; genetree spent roughly 350 min on the same computer, and it evaluated about 5 genealogies per second. However, migrate's genealogies are autocorrelated, whereas genetree's are independently sampled.
Example Data Set.
For a realworld example, we used aligned mtDNA control region domain I sequences of populations from the Nile valley, described by Krings et al. (32). The aligned sequences were taken from the compilation of Handt et al. (33). We chose the following three groups: Egypt (79 sequences), Nubia (69 sequences), and Sudan (79 sequences). These 225 sequences certainly violate several of the assumptions migrate is based on: the “populations” are assemblages of local populations, some or all of the population sizes were not constant, and migration rates between the populations were most likely not constant over time.
In our analysis, we ignored the existence of unsampled populations but took into account that mutation rates of the mtDNA control region Domain I data are heterogenous among sites by using a Hidden Markov Model with F84 mutation model with 4 rates (0.025, 0.239, 0.787, 2.354) with equal probability (34). This is an approximation to a Gamma distribution with α = 0.3 (see ref. 35). We used a transition–transversion ratio of 15 and the empirical base frequencies (A: 0.3302, C: 0.3313, G: 0.1161, T: 0.2225). We analyzed the data by using starting Θ_{i} of 0.5 and ℳ_{ji} of 5.0. Because of the size of the problem, we used a Metropoliscoupled MH algorithm (36) with four independent chains that accept at different rates. The chain that was used for the estimates uses an unmodified acceptance ratio, whereas the others accept more often. Switching between neighboring chains followed the approach of Kuhner and Felsenstein (37).
The results of the analysis are shown in Table 3. The gene flow among the populations seems to be moderate, except that there is considerable gene flow from Egypt into Nubia and from Sudan into Nubia.
Discussion
The present method allows us to analyze a wide range of different population models. It allows us to estimate as many as n population sizes Θ_{i} and n(n − 1) immigration rates ℳ_{ij} or as few as two parameters, a Θ that is equal for all subpopulations and an ℳ that is the same between all pairs of subpopulation. With the migration connection matrix, one can analyze arbitrary migration models where some migration routes are not allowed. This versatility allows us to consider biologically relevant migration scenarios and to put some of the complication under the control of the user.
The MH technique for this method is identical to that described in ref. 10. Our method wanders through the sample space by proposing local changes on a genealogy and rejecting or accepting such a changed genealogy according to their likelihood. These changes in genealogy are reversible: we showed (10) that this branch insertion and removal process allows us to connect any two genealogies with a modest number of rearrangements, and that genealogies are sampled in proportion to Prob(𝒢𝒫_{0}) Prob(𝒢𝒟). This MH sampler will converge to the correct answer when run for an infinitely long time, but of course our hope is that convergence will be achieved much earlier. For simple population scenarios, such as in our simulations of the nisland model, we can get similar parameter estimates even from bad starting parameters (Fig. 3). Our adaptive scheme using many short chains and a few long chains helps move the sampler into regions with genealogies that have high probabilities. As a result, estimates are more accurate than if they came from a single long chain run at an arbitrary 𝒫_{0}. For practical purposes, this selftargeting process for finding the appropriate distribution is important, as it seems less desirable to rely on the user to find good starting parameters or to ask the user to restart the estimation process many times.
For large data sets, the researcher may need to run migrate several times with different chain lengths to see whether the length and the number of the chains are influencing the result. Alternative strategies are the use of Metropolis coupling (36) (e.g., our realworld example) or summarizing over different chains (15, 38) or even over different runs. These extensions will improve results but will increase the time for analysis considerably. In genetree (11), which is currently the only competing coalescent likelihood program, no such adaptive scheme is used, and the researcher needs to find appropriate starting parameters by doing the iterations by hand. Our and Bahlo and Griffiths' schemes will produce strange estimates when the starting parameters are far from the truth (Fig. 3; ref. 31). Bayesian approaches, although they vary the parameters of interest during the sampling process, will have similar problems if they are based on MH: with increasing numbers of parameters, the search space gets larger and much more sampling needs to be done to produce a proper posterior distribution. So far, there is no Bayesian method for analyzing migration models by using the coalescent, except for the twopopulation method developed by Nielsen and Slatkin (39).
The comparison with genetree shows that for the cases chosen, both methods deliver similarly shaped likelihood surfaces, as they should, because both are approximating the same likelihood criterion (17). For this data set, genetree has slightly wider confidence intervals in the Θ direction than migrate (Fig. 4). When using only a single population, both programs deliver almost identical likelihood curves and therefore confidence intervals (data not shown). The small differences might be caused by the different assumptions about the mutation model or by the different distributions from which the programs sample their genealogies.
Advantages of migrate over the current version of genetree are that the researcher can take into account different mutation models, such as the infinite allele model, a stepwise mutation model for microsatellite data, and sequence evolution models with rate heterogeneity among sites (34). All models can be combined with a model of rate heterogeneity among loci (10).
In the simulations tests of an nisland migration model, the averages of the twoparameter model are rather close to the values used to generate the data sets but are most often slightly smaller. This is in stark contrast to theoretical results showing that expectations of parameters over all simulations do not exist or at least are highly biased upwards (41). There exists a very small but nonzero probability that the data are compatible with a genealogy of infinite length. If such a data set is encountered in a simulation study, the program will return very large parameter estimates. The distributions of the 100 10locus estimates from the simulation have heavy right tails (skewness S for Θ is 0.063 and for 4Nm is 0.11). The skewness is more pronounced if we look at the distribution of the 1,000 single locus estimates (S_{Θ} = 0.195, S_{4Nm} = 0.896). In fact, there is an upwards bias that is reflected in the skewness but not much in the averages because in these 100 simulation runs, we have not yet encountered a very high parameter value. On the other hand, there is a “fatal attraction” to zero: once a parameter becomes very small, it is unlikely that our adaptive MH procedure will succeed in reaching higher parameter values, because the events that are under the control of that parameter are not proposed and therefore subsequent parameter estimates tend to stay small.
For some population structures, such as a hidden source–sink scenario with large gene flow from a large population to a small population, results are not very enlightening without additional information about the migration structure. It remains to be shown that other methods are superior for these kinds of data. We expect that when we do not know the migration structure, many loci and many individuals will be needed to detect the few new mutations in the small sink population. Only then would we be able to see whether any of these rare mutations migrate back into the source population.
The difficulty of retrieving the true parameters increases with the number of parameters. Estimates for two parameters show smaller profile confidence intervals than the estimates with 16 parameters, but if the true population structure is complicated, the twoparameter model also delivers much less information than a more parameterrich model.
Krings et al. (32) infer gene flow in the Nile valley by using analyses of molecular variance (AMOVA) (40). Their findings coincide with ours, in that Nubia seems to have received a considerable number of genes from Egypt and Sudan. The population sizes are most likely inflated, because we did not take into account that the individual populations are substructured, and because all populations exchange migrants with their other neighbors, who contribute genetic variation which we do not account for in our analysis.
The rather large range of possible values for the migration parameters in the example of possible migration directions in the Nile valley makes it evident that single locus data, even if it is highly variable, does not help much in clarifying current discussions in anthropology. Additional unlinked loci, each with its own coalescent history, can reduce this uncertainty greatly (10, 17).
Conclusion
We have presented three lines of evidence that our method works even for rather complicated migration models: derivation of the MH sampling strategy (10, 15, 16), simulation to test convergence to true parameters, and simulations to assess biases and to see whether the method achieves results that are reproducible and can be found in a reasonable amount of time.
Maximum likelihood methods for the estimation of population parameters, as implemented in migrate or genetree, will make the current F_{ST}based estimators obsolete, because these do not take into account the genealogical relationship of the sample and the possibility of asymmetry in gene flow. However, the practical use of these new programs is limited to few subpopulations or few parameters because of current lack of computation power. Incorporation of the machinery of migrate into a program that can handle additional forces such as recombination and population growth is under way in our laboratory. The program migrate is available at http://evolution.genetics.washington.edu/lamarc.html.
Acknowledgments
We thank M. K. Kuhner and J. Yamato for many discussions and comments on the manuscript, and R. C. Griffiths and J. Wakeley for helpful comments on the manuscript. This work was funded by National Science Foundation grant no. BIR 9527687 and National Institutes of Health grants nos. R01 GM 51929, R01 GM 01989, all to J.F.
Footnotes

↵* To whom reprint requests should be addressed. Email: beerli{at}genetics.washington.edu.
Abbreviation
 MH,
 Metropolis–Hastings Markov chain Monte Carlo approach
 Accepted February 9, 2001.
 Copyright © 2001, The National Academy of Sciences
References
 ↵
 Wright S
 ↵
 Hudson R R,
 Slatkin M,
 Maddison W P
 ↵
 Slatkin M
 ↵
 Excoffier L,
 Smouse P E

 Weir B S
 ↵
 Rousset F
 ↵
 Carvalho G
 Beerli P
 ↵
 Gaggiotti O E,
 Excoffier L
 ↵
 Tufto J,
 Engen S,
 Hindar K
 ↵
 Beerli P,
 Felsenstein J
 ↵
 ↵
Wakeley, J. (2000) Theor. Popul. Biol., in press.
 ↵
 Gani J,
 Hannan E
 Kingman J
 ↵
 ↵
 Kuhner M K,
 Yamato J,
 Felsenstein J
 ↵
 Kuhner M K,
 Yamato J,
 Felsenstein J
 ↵
 Felsenstein J,
 Kuhner M K,
 Yamato J,
 Beerli P
 ↵
 Kuhner M K,
 Yamato J,
 Felsenstein J
 ↵
 Hillis D,
 Moritz C,
 Mable B
 Swofford D,
 Olsen G,
 Waddell P,
 Hillis D
 ↵
 ↵
 Hudson R R
 ↵
 Hammersley J M,
 Handscomb D C
 ↵
 ↵
 Gilks W R,
 Richardson S,
 Spiegelhalter D J
 ↵
 Wright S
 ↵
 ↵
 ↵
 ↵
 ↵
 ↵
 Stephens M,
 Donnelly P
 ↵
 ↵
 Handt O,
 Meyer S,
 von Haeseler A
 ↵
 ↵
 ↵
 Keramidas E M
 Geyer C
 ↵
 ↵
 Geyer C
 ↵
 ↵
 Excoffier L,
 Smouse P E,
 Quattro J M
 ↵
 Kuhner M K,
 Beerli P,
 Yamato J,
 Felsenstein J
Citation Manager Formats
More Articles of This Classification
Biological Sciences
Evolution
Related Content
 No related articles found.
Cited by...
 Shifting Quaternary migration patterns in the Bahamian archipelago: Evidence from the Zamia pumila complex at the northern limits of the Caribbean island biodiversity hotspot
 Phylogeography and genetic structure of endemic Acmispon argophyllus and A. dendroideus (Fabaceae) across the California Channel Islands
 Evolutionary history and gene flow of an endemic island oak: Quercus pacifica
 Phylogeographic model selection leads to insight into the evolutionary history of foureyed frogs
 Comparative phylogeography of black mangroves (Avicennia germinans) and red mangroves (Rhizophora mangle) in Florida: Testing the maritime discontinuity in coastal plants
 Postglacial expansion pathways of red mangrove, Rhizophora mangle, in the Caribbean Basin and Florida
 Understanding population structure and historical demography in a conservation context: Population genetics of the endangered Kirengeshoma palmata (Hydrangeaceae)
 Exegeses on Maximum Genetic Differentiation
 Functional Extinction of Birds Drives Rapid Evolutionary Changes in Seed Size
 Identifying Loci Under Selection Against Gene Flow in IsolationwithMigration Models
 Insights from population genetics for range limits of a widely distributed native plant
 A legacy of contrasting spatial genetic structure on either side of the AtlanticMediterranean transition zone in a marine protist
 Ancient Admixture in Human History
 Nuclear Genomic Sequences Reveal that Polar Bears Are an Old and Distinct Bear Lineage
 The rare peat moss Sphagnum wulfianum (Sphagnaceae) did not survive the last glacial period in northern European refugia
 Complex Population Dynamics and the Coalescent Under Neutrality
 Genetic consequences of seed banks in the perennial herb Arabidopsis lyrata subsp. petraea (Brassicaceae)
 Postglacial northsouth expansion of populations of Rhizophora mangle (Rhizophoraceae) along the Brazilian coast revealed by microsatellite analysis
 The Confounding Effects of Population Structure, Genetic Diversity and the Sampling Scheme on the Detection and Quantification of Population Size Changes
 Morphologically cryptic biological species within the liverwort Frullania asagrayana
 Bayesian species delimitation using multilocus sequence data
 Unified Framework to Evaluate Panmixia and Migration Direction Among Multiple Sampling Locations
 Spatiotemporal population structuring and genetic diversity retention in depleted Atlantic Bluefin tuna of the Mediterranean Sea
 Estimating Divergence Parameters With Small Samples From a Large Number of Loci
 Phylogenomics of primates and their ancestral populations
 Phylogeographic patterns and demographic history of Schiedea globosa (Caryophyllaceae) on the Hawaiian Islands
 Approximate Bayesian Computation Without Summary Statistics: The Case of Admixture
 Inference of Historical Changes in Migration Rate From the Lengths of Migrant Tracts
 A New Bayesian Method to Identify the Environmental Factors That Influence Recent Migration
 Estimation of the Population Scaled Mutation Rate From Microsatellite Data
 Joint Estimation of Migration Rate and Effective Population Size Using the Island Model
 Comparative Study of Methods for Detecting Sequence Compartmentalization in Human Immunodeficiency Virus Type 1
 Fast and Accurate Estimation of the PopulationScaled Mutation Rate, {theta}, From Microsatellite Genotype Data
 Highresolution species trees without concatenation
 Integration within the Felsenstein equation for improved Markov chain Monte Carlo methods in population genetics
 Comparing Likelihood and Bayesian Coalescent Estimation of Population Parameters
 Concordant Genetic Estimators of Migration Reveal Anthropogenically Enhanced SourceSink Population Structure in the River Sculpin, Cottus gobio
 Assessing the Effects of Human Mixing Patterns on Human Immunodeficiency Virus1 Interhost Phylogenetics Through Social Network Simulation.
 Phylogeographic History and Gene Flow Among Giant Galapagos Tortoises on Southern Isabela Island
 Interrogating multiple aspects of variation in a full resequencing data set to infer human population size changes
 Likelihoods From Summary Statistics: Recent Divergence Between Species
 Bayesian Estimation of Recent Migration Rates After a Spatial Expansion
 Microsatellite analysis reveals genetically distinct populations of red pine (Pinus resinosa, Pinaceae)
 The Structured Ancestral Selection Graph and the ManyDemes Limit
 Using Temporally Spaced Sequences to Simultaneously Estimate Migration Rates, Mutation Rate and Population Sizes in Measurably Evolving Populations
 MaximumLikelihood Estimation of Demographic Parameters Using the Frequency Spectrum of Unlinked SingleNucleotide Polymorphisms
 Controlling for the Effects of History and Nonequilibrium Conditions in Gene Flow Estimates in Northern Bullfrog (Rana catesbeiana) Populations
 The Molecular Population Genetics of HIV1 Group O
 Recombination and Migration of Cryphonectria hypovirus 1 as Inferred From Gene Genealogies and the Coalescent
 Population Subdivision and Molecular Sequence Variation: Theory and Analysis of Drosophila ananassae Data
 Testing for Asymmetrical Gene Flow in a Drosophila melanogaster BodySize Cline
 Distinct geographic patterns of genetic diversity are maintained in wild barley (Hordeum vulgare ssp. spontaneum) despite migration
 Bayes Estimation of Species Divergence Times and Ancestral Population Sizes Using DNA Sequences From Multiple Loci
 Whales Before Whaling in the North Atlantic
 Estimation of Population Growth or Decline in Genetically Monitored Populations
 Evolutionary Indicators of Human Immunodeficiency Virus Type 1 Reservoirs and Compartments
 Bayesian Inference of Recent Migration Rates Using Multilocus Genotypes
 Estimating Effective Population Size and Migration Rates From Genetic Samples Over Space and Time
 Genetic Effective Size Is Three Orders of Magnitude Smaller Than Adult Census Size in an Abundant, EstuarineDependent Marine Fish (Sciaenops ocellatus)
 Estimating Mutation Parameters, Population History and Genealogy Simultaneously From Temporally Spaced Sequence Data