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Research Article

Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum

Sorel T. Fitz-Gibbon, Heidi Ladner, Ung-Jin Kim, Karl O. Stetter, Melvin I. Simon, and Jeffrey H. Miller
PNAS January 22, 2002 99 (2) 984-989; https://doi.org/10.1073/pnas.241636498
Sorel T. Fitz-Gibbon
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Heidi Ladner
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Ung-Jin Kim
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Karl O. Stetter
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Melvin I. Simon
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Jeffrey H. Miller
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  1. Contributed by Melvin I. Simon

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Abstract

We determined and annotated the complete 2.2-megabase genome sequence of Pyrobaculum aerophilum, a facultatively aerobic nitrate-reducing hyperthermophilic (Topt = 100°C) crenarchaeon. Clues were found suggesting explanations of the organism's surprising intolerance to sulfur, which may aid in the development of methods for genetic studies of the organism. Many interesting features worthy of further genetic studies were revealed. Whole genome computational analysis confirmed experiments showing that P. aerophilum (and perhaps all crenarchaea) lack 5′ untranslated regions in their mRNAs and thus appear not to use a ribosome-binding site (Shine–Dalgarno)-based mechanism for translation initiation at the 5′ end of transcripts. Inspection of the lengths and distribution of mononucleotide repeat-tracts revealed some interesting features. For instance, it was seen that mononucleotide repeat-tracts of Gs (or Cs) are highly unstable, a pattern expected for an organism deficient in mismatch repair. This result, together with an independent study on mutation rates, suggests a “mutator” phenotype.

Footnotes

    • ↵‡ Present address: School of Medicine, University of California, Davis, CA 95616.

    • ↵‖ To whom reprint requests should be addressed. E-mail: jhmiller{at}mbi.ucla.edu.

    • Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. AE009441).

    • Accepted November 30, 2001.
    • Copyright © 2002, The National Academy of Sciences
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    Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum
    Sorel T. Fitz-Gibbon, Heidi Ladner, Ung-Jin Kim, Karl O. Stetter, Melvin I. Simon, Jeffrey H. Miller
    Proceedings of the National Academy of Sciences Jan 2002, 99 (2) 984-989; DOI: 10.1073/pnas.241636498

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    Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum
    Sorel T. Fitz-Gibbon, Heidi Ladner, Ung-Jin Kim, Karl O. Stetter, Melvin I. Simon, Jeffrey H. Miller
    Proceedings of the National Academy of Sciences Jan 2002, 99 (2) 984-989; DOI: 10.1073/pnas.241636498
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    Proceedings of the National Academy of Sciences: 99 (2)
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