Time of feeding and the intrinsic circadian clock drive rhythms in hepatic gene expression

Christopher  Vollmers; Shubhroz  Gill; Luciano  Di Tacchio; Sandhya R.  Pulivarthy; Hiep D.  Le; Satchidananda  Panda

doi:10.1073/pnas.0909591106

New Research In

Edited by Ronald M. Evans, The Salk Institute for Biological Studies, La Jolla, CA, and approved October 13, 2009 (received for review August 24, 2009)

Abstract

In mammals, the circadian oscillator generates approximately 24-h rhythms in feeding behavior, even under constant environmental conditions. Livers of mice held under constant darkness exhibit circadian rhythm in abundance in up to 15% of expressed transcripts. Therefore, oscillations in hepatic transcripts could be driven by rhythmic food intake or sustained by the hepatic circadian oscillator, or a combination of both. To address this question, we used distinct feeding and fasting paradigms on wild-type (WT) and circadian clock-deficient mice. We monitored temporal patterns of feeding and hepatic transcription. Both food availability and the temporal pattern of feeding determined the repertoire, phase, and amplitude of the circadian transcriptome in WT liver. In the absence of feeding, only a small subset of transcripts continued to express circadian patterns. Conversely, temporally restricted feeding restored rhythmic transcription of hundreds of genes in oscillator-deficient mouse liver. Our findings show that both temporal pattern of food intake and the circadian clock drive rhythmic transcription, thereby highlighting temporal regulation of hepatic transcription as an emergent property of the circadian system.

Footnotes

¹To whom correspondence should be addressed. E-mail: panda{at}salk.edu
Author contributions: C.V., L.D., and S.P. designed research; C.V., S.R.P., and H.D.L. performed research; C.V., S.G., and S.P. analyzed data; and C.V. and S.P. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.