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Surgery with molecular fluorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survival

Quyen T. Nguyen, Emilia S. Olson, Todd A. Aguilera, Tao Jiang, Miriam Scadeng, Lesley G. Ellies, and Roger Y. Tsien
PNAS published ahead of print February 16, 2010 https://doi.org/10.1073/pnas.0910261107
Quyen T. Nguyen
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Emilia S. Olson
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Todd A. Aguilera
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Tao Jiang
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Miriam Scadeng
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Lesley G. Ellies
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Roger Y. Tsien
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  1. Edited* by Robert Langer, Massachusetts Institute of Technology, Cambridge, MA, and approved December 24, 2009 (received for review September 9, 2009)

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Abstract

The completeness of tumor removal during surgery is dependent on the surgeon’s ability to differentiate tumor from normal tissue using subjective criteria that are not easily quantifiable. A way to objectively assess tumor margins during surgery in patients would be of great value. We have developed a method to visualize tumors during surgery using activatable cell-penetrating peptides (ACPPs), in which the fluorescently labeled, polycationic cell-penetrating peptide (CPP) is coupled via a cleavable linker to a neutralizing peptide. Upon exposure to proteases characteristic of tumor tissue, the linker is cleaved, dissociating the inhibitory peptide and allowing the CPP to bind to and enter tumor cells. In mice, xenografts stably transfected with green fluorescent protein show colocalization with the Cy5-labeled ACPPs. In the same mouse models, Cy5-labeled free ACPPs and ACPPs conjugated to dendrimers (ACPPDs) delineate the margin between tumor and adjacent tissue, resulting in improved precision of tumor resection. Surgery guided by ACPPD resulted in fewer residual cancer cells left in the animal after surgery as measured by Alu PCR. A single injection of ACPPD dually labeled with Cy5 and gadolinium chelates enabled preoperative whole-body tumor detection by MRI, intraoperative guidance by real-time fluorescence, intraoperative histological analysis of margin status by fluorescence, and postoperative MRI tumor quantification. Animals whose tumors were resected with ACPPD guidance had better long-term tumor-free survival and overall survival than animals whose tumors were resected with traditional bright-field illumination only.

  • intraoperative fluorescence imaging
  • molecular navigation
  • long-term survival
  • molecular imaging
  • surgical margin

Footnotes

  • 1To whom correspondence should be addressed. E-mail: rtsien{at}ucsd.edu.
  • Author contributions: Q.T.N., E.S.O., T.A.A., and R.Y.T. designed research; Q.T.N., E.S.O., T.A.A., and M.S. performed research; T.J. and L.G.E. contributed new reagents/analytic tools; Q.T.N., E.S.O., T.A.A., and R.Y.T. analyzed data; and Q.T.N. and E.S.O. wrote the paper.

  • Conflict of interest statement: Q.T.N., E.S.O., T.A.A., T.J., and R.Y.T. have signed a scientific advisory agreement with a company founded to develop the technology described in this article.

  • ↵*This Direct Submission article had a prearranged editor.

  • This article contains supporting information online at www.pnas.org/cgi/content/full/0910261107/DCSupplemental.

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Surgery with molecular fluorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survival
Quyen T. Nguyen, Emilia S. Olson, Todd A. Aguilera, Tao Jiang, Miriam Scadeng, Lesley G. Ellies, Roger Y. Tsien
Proceedings of the National Academy of Sciences Feb 2010, 200910261; DOI: 10.1073/pnas.0910261107

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Surgery with molecular fluorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survival
Quyen T. Nguyen, Emilia S. Olson, Todd A. Aguilera, Tao Jiang, Miriam Scadeng, Lesley G. Ellies, Roger Y. Tsien
Proceedings of the National Academy of Sciences Feb 2010, 200910261; DOI: 10.1073/pnas.0910261107
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