Strong reversible Fe3+-mediated bridging between dopa-containing protein films in water

Hongbo Zeng; Dong Soo Hwang; Jacob N. Israelachvili; J. Herbert Waite

doi:10.1073/pnas.1007416107

New Research In

Contributed by Jacob N. Israelachvili, June 1, 2010 (sent for review February 10, 2010)
↵¹H.Z. and D.S.H. contributed equally to this work.

Abstract

Metal-containing polymer networks are widespread in biology, particularly for load-bearing exoskeletal biomaterials. Mytilus byssal cuticle is an especially interesting case containing moderate levels of Fe³⁺ and cuticle protein—mussel foot protein-1 (mfp-1), which has a peculiar combination of high hardness and high extensibility. Mfp-1, containing 13 mol % of dopa (3, 4-dihydroxyphenylalanine) side-chains, is highly positively charged polyelectrolyte (pI ∼ 10) and didn’t show any cohesive tendencies in previous surface forces apparatus (SFA) studies. Here, we show that Fe³⁺ ions can mediate unusually strong interactions between the positively charged proteins. Using an SFA, Fe³⁺ was observed to impart robust bridging (W_ad ≈ 4.3 mJ/m²) between two noninteracting mfp-1 films in aqueous buffer approaching the ionic strength of seawater. The Fe³⁺ bridging between the mfp-1-coated surfaces is fully reversible in water, increasing with contact time and iron concentration up to 10 μM; at 100 μM, Fe³⁺ bridging adhesion is abolished. Bridging is apparently due to the formation of multivalent dopa-iron complexes. Similar Fe-mediated bridging (W_ad ≈ 5.7 mJ/m²) by a smaller recombinant dopa-containing analogue indicates that bridging is largely independent of molecular weight and posttranslational modifications other than dopa. The results suggest that dopa-metal interactions may provide an energetic new paradigm for engineering strong, self-healing interactions between polymers under water.

Footnotes

²To whom correspondence may be addressed. E-mail: hongbo.zeng{at}ualberta.ca, waite{at}lifesci.ucsb.edu, or jacob{at}engineering.ucsb.edu.
Author contributions: H.Z., D.S.H., J.N.I., and J.H.W. designed research; H.Z. and D.S.H. performed research; J.N.I. and J.H.W. contributed new reagents/analytic tools; H.Z. and D.S.H. analyzed data; and H.Z., D.S.H., J.N.I., and J.H.W. wrote the paper.
The authors declare no conflict of interest.
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