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Research Article

Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber

Subhra Chakraborty, Niranjan Chakraborty, Lalit Agrawal, Sudip Ghosh, Kanika Narula, Shubhendu Shekhar, Prakash S. Naik, P. C. Pande, Swarup Kumar Chakrborti, and Asis Datta
  1. aNational Institute of Plant Genome Research, New Delhi 110067, India;
  2. bCentral Potato Research Institute, Shimla, Himachal Pradesh 171001, India; and
  3. cCentral Potato Research Institute Campus, Modipuram, Uttar Pradesh 250110, India

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PNAS first published September 20, 2010; https://doi.org/10.1073/pnas.1006265107
Subhra Chakraborty
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  • For correspondence: subhrac@hotmail.com asisdatta@hotmail.com
Niranjan Chakraborty
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Lalit Agrawal
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Sudip Ghosh
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Kanika Narula
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Shubhendu Shekhar
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Prakash S. Naik
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P. C. Pande
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Swarup Kumar Chakrborti
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Asis Datta
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  • For correspondence: subhrac@hotmail.com asisdatta@hotmail.com
  1. Edited* by Eugene W. Nester, University of Washington, Seattle, WA, and approved August 17, 2010 (received for review May 5, 2010)

  2. ↵1S.C. and N.C. contributed equally to this article.

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Abstract

Protein deficiency is the most crucial factor that affects physical growth and development and that increases morbidity and mortality especially in developing countries. Efforts have been made to improve protein quality and quantity in crop plants but with limited success. Here, we report the development of transgenic potatoes with enhanced nutritive value by tuber-specific expression of a seed protein, AmA1 (Amaranth Albumin 1), in seven genotypic backgrounds suitable for cultivation in different agro-climatic regions. Analyses of the transgenic tubers revealed up to 60% increase in total protein content. In addition, the concentrations of several essential amino acids were increased significantly in transgenic tubers, which are otherwise limited in potato. Moreover, the transgenics also exhibited enhanced photosynthetic activity with a concomitant increase in total biomass. These results are striking because this genetic manipulation also resulted in a moderate increase in tuber yield. The comparative protein profiling suggests that the proteome rebalancing might cause increased protein content in transgenic tubers. Furthermore, the data on field performance and safety evaluation indicate that the transgenic potatoes are suitable for commercial cultivation. In vitro and in vivo studies on experimental animals demonstrate that the transgenic tubers are also safe for human consumption. Altogether, these results emphasize that the expression of AmA1 is a potential strategy for the nutritional improvement of food crops.

  • allergenecity
  • essential amino acids
  • nutritional health

Footnotes

  • 2To whom correspondence may be addressed. E-mail: subhrac{at}hotmail.com or asisdatta{at}hotmail.com.
  • Author contributions: S.C., N.C., and A.D. designed research; S.C., N.C., L.A., S.G., K.N., S.S., P.S.N., P.C.P., and S.K.C. performed research; S.C., N.C., L.A., S.G., K.N., S.S., P.S.N., P.C.P., S.K.C., and A.D. analyzed data; and S.C., N.C., and A.D. wrote the paper.

  • The authors declare no conflict of interest.

  • ↵*This Direct Submission article had a prearranged editor.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1006265107/-/DCSupplemental.

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    Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber
    Subhra Chakraborty, Niranjan Chakraborty, Lalit Agrawal, Sudip Ghosh, Kanika Narula, Shubhendu Shekhar, Prakash S. Naik, P. C. Pande, Swarup Kumar Chakrborti, Asis Datta
    Proceedings of the National Academy of Sciences Sep 2010, 201006265; DOI: 10.1073/pnas.1006265107

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    Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber
    Subhra Chakraborty, Niranjan Chakraborty, Lalit Agrawal, Sudip Ghosh, Kanika Narula, Shubhendu Shekhar, Prakash S. Naik, P. C. Pande, Swarup Kumar Chakrborti, Asis Datta
    Proceedings of the National Academy of Sciences Sep 2010, 201006265; DOI: 10.1073/pnas.1006265107
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