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Research Article

Clone history shapes Populus drought responses

Sherosha Raj, Katharina Bräutigam, Erin T. Hamanishi, Olivia Wilkins, Barb R. Thomas, William Schroeder, Shawn D. Mansfield, Aine L. Plant, and Malcolm M. Campbell
  1. aDepartment of Cell & Systems Biology,
  2. bCentre for the Analysis of Genome Evolution & Function, and
  3. cFaculty of Forestry, University of Toronto, Toronto, ON, Canada M5S 3B2;
  4. dAlberta-Pacific Forest Industries, Boyle, AB, Canada T0A 0M0;
  5. eDepartment of Renewable Resources, University of Alberta, Edmonton, AB, Canada T6G 2H1;
  6. fAgroforestry Development Centre, Agriculture and Agri-Food Canada, Indian Head, SK, Canada S0G 2K0;
  7. gDepartment of Wood Science, University of British Columbia, Vancouver, BC, Canada V6T 1Z4;
  8. hDepartment of Biological Sciences, Simon Fraser University, Burnaby, BC V5A 1S6, Canada; and
  9. iDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, ON, Canada M1C 1A4

See allHide authors and affiliations

PNAS first published July 11, 2011; https://doi.org/10.1073/pnas.1103341108
Sherosha Raj
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Katharina Bräutigam
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Erin T. Hamanishi
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Olivia Wilkins
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Barb R. Thomas
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William Schroeder
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Shawn D. Mansfield
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Aine L. Plant
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Malcolm M. Campbell
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  • For correspondence: malcolm.campbell@utoronto.ca
  1. Edited by Ronald R. Sederoff, North Carolina State University, Raleigh, NC, and approved May 25, 2011 (received for review March 1, 2011)

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Abstract

Just as animal monozygotic twins can experience different environmental conditions by being reared apart, individual genetically identical trees of the genus Populus can also be exposed to contrasting environmental conditions by being grown in different locations. As such, clonally propagated Populus trees provide an opportunity to interrogate the impact of individual environmental history on current response to environmental stimuli. To test the hypothesis that current responses to an environmental stimulus, drought, are contingent on environmental history, the transcriptome- level drought responses of three economically important hybrid genotypes—DN34 (Populus deltoides × Populus nigra), Walker [P. deltoides var. occidentalis × (Populus laurifolia × P. nigra)], and Okanese [Walker × (P. laurifolia × P. nigra)]—derived from two different locations were compared. Strikingly, differences in transcript abundance patterns in response to drought were based on differences in geographic origin of clones for two of the three genotypes. This observation was most pronounced for the genotypes with the longest time since establishment and last common propagation. Differences in genome-wide DNA methylation paralleled the transcriptome level trends, whereby the clones with the most divergent transcriptomes and clone history had the most marked differences in the extent of total DNA methylation, suggesting an epigenomic basis for the clone history-dependent transcriptome divergence. The data provide insights into the interplay between genotype and environment in the ecologically and economically important Populus genus, with implications for the industrial application of Populus trees and the evolution and persistence of these important tree species and their associated hybrids.

  • epigenetics
  • forest trees
  • poplar

Footnotes

  • ↵1S.R. and K.B. contributed equally to this work.

  • ↵2To whom correspondence should be addressed. E-mail: malcolm.campbell{at}utoronto.ca.
  • Author contributions: S.R., S.D.M., A.L.P., and M.M.C. designed research; S.R., K.B., E.T.H., and O.W. performed research; B.R.T. and W.S. contributed new reagents/analytic tools; S.R., K.B., O.W., and M.M.C. analyzed data; and S.R., K.B., and M.M.C. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • Data deposition: The data reported in this paper have been deposited in the Gene Expression Omnibus (GEO) database, www.ncbi.nlm.nih.gov/geo (accession no. GSE27693).

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1103341108/-/DCSupplemental.

Freely available online through the PNAS open access option.

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Clone history shapes Populus drought responses
Sherosha Raj, Katharina Bräutigam, Erin T. Hamanishi, Olivia Wilkins, Barb R. Thomas, William Schroeder, Shawn D. Mansfield, Aine L. Plant, Malcolm M. Campbell
Proceedings of the National Academy of Sciences Jul 2011, 201103341; DOI: 10.1073/pnas.1103341108

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Clone history shapes Populus drought responses
Sherosha Raj, Katharina Bräutigam, Erin T. Hamanishi, Olivia Wilkins, Barb R. Thomas, William Schroeder, Shawn D. Mansfield, Aine L. Plant, Malcolm M. Campbell
Proceedings of the National Academy of Sciences Jul 2011, 201103341; DOI: 10.1073/pnas.1103341108
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