Researchers are exploring whether these ubiquitous fluorinated molecules might worsen infections or hamper vaccine effectiveness.
Image credit: Shutterstock/Dmitry Naumov.
Edited by Laurie H. Glimcher, Weill Cornell Medical College, New York, New York, and approved January 20, 2012 (received for review July 15, 2011)
Abstract
Despite the importance of Salmonella infections in human and animal health, the target antigens of Salmonella-specific immunity remain poorly defined. We have previously shown evidence for antibody-mediating protection against invasive Salmonellosis in mice and African children. To generate an overview of antibody targeting in systemic Salmonellosis, a Salmonella proteomic array containing over 2,700 proteins was constructed and probed with immune sera from Salmonella-infected mice and humans. Analysis of multiple inbred mouse strains identified 117 antigens recognized by systemic antibody responses in murine Salmonellosis. Importantly, many of these antigens were independently identified as target antigens using sera from Malawian children with Salmonella bacteremia, validating the study of the murine model. Furthermore, vaccination with SseB, the most prominent antigenic target in Malawian children, provided mice with significant protection against Salmonella infection. Together, these data uncover an overlapping immune signature of disseminated Salmonellosis in mice and humans and provide a foundation for the generation of a protective subunit vaccine.
Footnotes
- ↵1To whom correspondence should be addressed. E-mail: sjmcsorley{at}ucdavis.edu.
Author contributions: R.S.H., C.A.M., P.L.F., D.H.D., and S.J.M. designed research; S.-J.L., L.L., S.J., M.R.N., E.N.G., C.L.M., M.A.K., F.N., J.N.H., P.H., R.M.T., and D.H.D. performed research; L.L., R.S.H., P.L.F., and D.H.D. contributed new reagents/analytic tools; S.-J.L., L.L., R.M.T., C.A.M., P.L.F., D.H.D., and S.J.M. analyzed data; and C.A.M., D.H.D., and S.J.M. wrote the paper.
Conflict of interest statement: C.A.M. and F.N. are both employees of the Novartis Vaccines Institute for Global Health. C.A.M. has received grant support from GlaxoSmithKline.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1111413109/-/DCSupplemental.
Identification of a common immune signature in murine and human systemic Salmonellosis
Sign up for Article Alerts
Jump to section
You May Also be Interested in
Recent experiments and simulations are starting to answer some fundamental questions about how life came to be.
Image credit: Shutterstock/Radoslaw Lecyk.
Archaeologists have long tried to define the transition between the two time periods.
Image credit: Ceri Shipton.
Adam Mastroianni and Daniel Gilbert explore why conversations almost never end when people want them to.
A study finds that giant pandas roll in horse manure to increase their cold tolerance.
Image credit: Fuwen Wei.