A distinct lineage of influenza A virus from bats

Suxiang Tong; Yan Li; Pierre Rivailler; Christina Conrardy; Danilo A. Alvarez Castillo; Li-Mei Chen; Sergio Recuenco; James A. Ellison; Charles T. Davis; Ian A. York; Amy S. Turmelle; David Moran; Shannon Rogers; Mang Shi; Ying Tao; Michael R. Weil; Kevin Tang; Lori A. Rowe; Scott Sammons; Xiyan Xu; Michael Frace; Kim A. Lindblade; Nancy J. Cox; Larry J. Anderson; Charles E. Rupprecht; Ruben O. Donis

doi:10.1073/pnas.1116200109

New Research In

Edited by Robert A. Lamb, Northwestern University, Evanston, IL, and approved January 17, 2012 (received for review October 7, 2011)

Abstract

Influenza A virus reservoirs in animals have provided novel genetic elements leading to the emergence of global pandemics in humans. Most influenza A viruses circulate in waterfowl, but those that infect mammalian hosts are thought to pose the greatest risk for zoonotic spread to humans and the generation of pandemic or panzootic viruses. We have identified an influenza A virus from little yellow-shouldered bats captured at two locations in Guatemala. It is significantly divergent from known influenza A viruses. The HA of the bat virus was estimated to have diverged at roughly the same time as the known subtypes of HA and was designated as H17. The neuraminidase (NA) gene is highly divergent from all known influenza NAs, and the internal genes from the bat virus diverged from those of known influenza A viruses before the estimated divergence of the known influenza A internal gene lineages. Attempts to propagate this virus in cell cultures and chicken embryos were unsuccessful, suggesting distinct requirements compared with known influenza viruses. Despite its divergence from known influenza A viruses, the bat virus is compatible for genetic exchange with human influenza viruses in human cells, suggesting the potential capability for reassortment and contributions to new pandemic or panzootic influenza A viruses.

Footnotes

↵¹To whom correspondence may be addressed. E-mail: sot1{at}cdc.gov, cyr5{at}cdc.gov, or rvd6{at}cdc.gov.

Author contributions: S.T., C.E.R., and R.O.D. designed research; S.T., Y.L., P.R., C.C., D.A.A.C., L.-M.C., S. Recuenco, J.A.E., C.T.D., I.A.Y., A.S.T., D.M., M.S., Y.T., M.R.W., K.T., L.A.R., S.S., and X.X. performed research; S.T., Y.L., P.R., L.-M.C., I.A.Y., S. Rogers, M.S., C.E.R., and R.O.D. contributed new reagents/analytic tools; S.T., Y.L., P.R., C.C., D.A.A.C., L.-M.C., S. Recuenco, J.A.E., C.T.D., I.A.Y., A.S.T., D.M., M.S., Y.T., M.R.W., K.T., L.A.R., S.S., X.X., M.F., K.A.L., N.J.C., L.J.A., C.E.R., and R.O.D. analyzed data; and S.T., Y.L., P.R., L.-M.C., C.T.D., I.A.Y., M.S., M.R.W., L.J.A., C.E.R., and R.O.D. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. CY103873, CY103874, CY103875, CY103876, CY103877, CY103878, CY103879, CY103880, CY103881, CY103882, CY103883, CY103884, CY103885, CY103886, CY103887, CY103888, CY103889, CY103890, CY103891, CY103892, CY103893, CY103894, CY103895, and CY103896).
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