Skip to main content

Main menu

  • Home
  • Articles
    • Current
    • Special Feature Articles - Most Recent
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • List of Issues
  • Front Matter
    • Front Matter Portal
    • Journal Club
  • News
    • For the Press
    • This Week In PNAS
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Editorial and Journal Policies
    • Submission Procedures
    • Fees and Licenses
  • Submit
  • Submit
  • About
    • Editorial Board
    • PNAS Staff
    • FAQ
    • Accessibility Statement
    • Rights and Permissions
    • Site Map
  • Contact
  • Journal Club
  • Subscribe
    • Subscription Rates
    • Subscriptions FAQ
    • Open Access
    • Recommend PNAS to Your Librarian

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Home
Home
  • Log in
  • My Cart

Advanced Search

  • Home
  • Articles
    • Current
    • Special Feature Articles - Most Recent
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • List of Issues
  • Front Matter
    • Front Matter Portal
    • Journal Club
  • News
    • For the Press
    • This Week In PNAS
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Editorial and Journal Policies
    • Submission Procedures
    • Fees and Licenses
  • Submit
Research Article

High-throughput single-microparticle imaging flow analyzer

Keisuke Goda, Ali Ayazi, Daniel R. Gossett, Jagannath Sadasivam, Cejo K. Lonappan, Elodie Sollier, Ali M. Fard, Soojung Claire Hur, Jost Adam, Coleman Murray, Chao Wang, Nora Brackbill, Dino Di Carlo, and Bahram Jalali
  1. aDepartment of Electrical Engineering, University of California, Los Angeles, CA 90095;
  2. bCalifornia NanoSystems Institute, Los Angeles, CA 90095;
  3. cDepartment of Bioengineering, University of California, Los Angeles, CA 90095;
  4. dDepartment of Mechanical and Aerospace Engineering, University of California, Los Angeles, CA 90095;
  5. eDepartment of Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA 90095; and
  6. fEli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California, Los Angeles, CA 90095

See allHide authors and affiliations

PNAS first published July 2, 2012; https://doi.org/10.1073/pnas.1204718109
Keisuke Goda
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: goda@ee.ucla.edu
Ali Ayazi
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel R. Gossett
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jagannath Sadasivam
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cejo K. Lonappan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elodie Sollier
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ali M. Fard
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Soojung Claire Hur
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jost Adam
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Coleman Murray
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chao Wang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nora Brackbill
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dino Di Carlo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bahram Jalali
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  1. Edited by David A. Weitz, Harvard University, Cambridge, MA, and approved June 6, 2012 (received for review March 22, 2012)

  • Article
  • Figures & SI
  • Info & Metrics
  • PDF
Loading

Abstract

Optical microscopy is one of the most widely used diagnostic methods in scientific, industrial, and biomedical applications. However, while useful for detailed examination of a small number (< 10,000) of microscopic entities, conventional optical microscopy is incapable of statistically relevant screening of large populations (> 100,000,000) with high precision due to its low throughput and limited digital memory size. We present an automated flow-through single-particle optical microscope that overcomes this limitation by performing sensitive blur-free image acquisition and nonstop real-time image-recording and classification of microparticles during high-speed flow. This is made possible by integrating ultrafast optical imaging technology, self-focusing microfluidic technology, optoelectronic communication technology, and information technology. To show the system’s utility, we demonstrate high-throughput image-based screening of budding yeast and rare breast cancer cells in blood with an unprecedented throughput of 100,000 particles/s and a record false positive rate of one in a million.

  • photonics
  • microfluidics
  • instrumentation
  • high-throughput screening
  • medical diagnostics

Footnotes

  • ↵1To whom correspondence should be addressed. E-mail: goda{at}ee.ucla.edu.
  • Author contributions: K.G. designed research; K.G., A.A., D.R.G., J.S., C.K.L., E.S., C.W., and N.B. performed research; K.G., D.R.G., J.S., C.K.L., E.S., A.M.F., S.C.H., and C.M. contributed new reagents/analytic tools; K.G., A.A., J.S., C.K.L., A.M.F., J.A., and C.W. analyzed data; and K.G., A.A., D.R.G., D.D.C., and B.J. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1204718109/-/DCSupplemental.

Freely available online through the PNAS open access option.

Next
Back to top
Article Alerts
Email Article

Thank you for your interest in spreading the word on PNAS.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
High-throughput single-microparticle imaging flow analyzer
(Your Name) has sent you a message from PNAS
(Your Name) thought you would like to see the PNAS web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
High-throughput imaging flow analyzer
Keisuke Goda, Ali Ayazi, Daniel R. Gossett, Jagannath Sadasivam, Cejo K. Lonappan, Elodie Sollier, Ali M. Fard, Soojung Claire Hur, Jost Adam, Coleman Murray, Chao Wang, Nora Brackbill, Dino Di Carlo, Bahram Jalali
Proceedings of the National Academy of Sciences Jul 2012, DOI: 10.1073/pnas.1204718109

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
High-throughput imaging flow analyzer
Keisuke Goda, Ali Ayazi, Daniel R. Gossett, Jagannath Sadasivam, Cejo K. Lonappan, Elodie Sollier, Ali M. Fard, Soojung Claire Hur, Jost Adam, Coleman Murray, Chao Wang, Nora Brackbill, Dino Di Carlo, Bahram Jalali
Proceedings of the National Academy of Sciences Jul 2012, DOI: 10.1073/pnas.1204718109
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Mendeley logo Mendeley
Proceedings of the National Academy of Sciences: 118 (9)
Current Issue

Submit

Sign up for Article Alerts

Jump to section

  • Article
  • Figures & SI
  • Info & Metrics
  • PDF

You May Also be Interested in

Setting sun over a sun-baked dirt landscape
Core Concept: Popular integrated assessment climate policy models have key caveats
Better explicating the strengths and shortcomings of these models will help refine projections and improve transparency in the years ahead.
Image credit: Witsawat.S.
Model of the Amazon forest
News Feature: A sea in the Amazon
Did the Caribbean sweep into the western Amazon millions of years ago, shaping the region’s rich biodiversity?
Image credit: Tacio Cordeiro Bicudo (University of São Paulo, São Paulo, Brazil), Victor Sacek (University of São Paulo, São Paulo, Brazil), and Lucy Reading-Ikkanda (artist).
Syrian archaeological site
Journal Club: In Mesopotamia, early cities may have faltered before climate-driven collapse
Settlements 4,200 years ago may have suffered from overpopulation before drought and lower temperatures ultimately made them unsustainable.
Image credit: Andrea Ricci.
Steamboat Geyser eruption.
Eruption of Steamboat Geyser
Mara Reed and Michael Manga explore why Yellowstone's Steamboat Geyser resumed erupting in 2018.
Listen
Past PodcastsSubscribe
Birds nestling on tree branches
Parent–offspring conflict in songbird fledging
Some songbird parents might improve their own fitness by manipulating their offspring into leaving the nest early, at the cost of fledgling survival, a study finds.
Image credit: Gil Eckrich (photographer).

Similar Articles

Site Logo
Powered by HighWire
  • Submit Manuscript
  • Twitter
  • Facebook
  • RSS Feeds
  • Email Alerts

Articles

  • Current Issue
  • Special Feature Articles – Most Recent
  • List of Issues

PNAS Portals

  • Anthropology
  • Chemistry
  • Classics
  • Front Matter
  • Physics
  • Sustainability Science
  • Teaching Resources

Information

  • Authors
  • Editorial Board
  • Reviewers
  • Subscribers
  • Librarians
  • Press
  • Site Map
  • PNAS Updates
  • FAQs
  • Accessibility Statement
  • Rights & Permissions
  • About
  • Contact

Feedback    Privacy/Legal

Copyright © 2021 National Academy of Sciences. Online ISSN 1091-6490