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Research Article

Pubertally born neurons and glia are functionally integrated into limbic and hypothalamic circuits of the male Syrian hamster

Margaret A. Mohr and Cheryl L. Sisk
PNAS first published March 4, 2013; https://doi.org/10.1073/pnas.1219443110
Margaret A. Mohr
aNeuroscience Program, and
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  • For correspondence: mohrmarg@msu.edu
Cheryl L. Sisk
aNeuroscience Program, and
bDepartment of Psychology, Michigan State University, East Lansing, MI 48824
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  1. Edited by Bruce S. McEwen, The Rockefeller University, New York, NY, and approved February 11, 2013 (received for review November 7, 2012)

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Abstract

During puberty, the brain goes through extensive remodeling, involving the addition of new neurons and glia to brain regions beyond the canonical neurogenic regions (i.e., dentate gyrus and olfactory bulb), including limbic and hypothalamic cell groups associated with sex-typical behavior. Whether these pubertally born cells become functionally integrated into neural circuits remains unknown. To address this question, we gave male Syrian hamsters daily injections of the cell birthdate marker bromodeoxyuridine throughout puberty (postnatal day 28–49). Half of the animals were housed in enriched environments with access to a running wheel to determine whether enrichment increased the survival of pubertally born cells compared with the control environment. At 4 wk after the last BrdU injection, animals were allowed to interact with a receptive female and were then killed 1 h later. Triple-label immunofluorescence for BrdU, the mature neuron marker neuronal nuclear antigen, and the astrocytic marker glial fibrillary acidic protein revealed that a proportion of pubertally born cells in the medial preoptic area, arcuate nucleus, and medial amygdala differentiate into either mature neurons or astrocytes. Double-label immunofluorescence for BrdU and the protein Fos revealed that a subset of pubertally born cells in these regions is activated during sociosexual behavior, indicative of their functional incorporation into neural circuits. Enrichment affected the survival and activation of pubertally born cells in a brain region-specific manner. These results demonstrate that pubertally born cells located outside of the traditional neurogenic regions differentiate into neurons and glia and become functionally incorporated into neural circuits that subserve sex-typical behaviors.

  • adolescence
  • gliogenesis
  • neurogenesis

Footnotes

  • ↵1To whom correspondence should be addressed. E-mail: mohrmarg{at}msu.edu.
  • Author contributions: M.A.M. and C.L.S. designed research; M.A.M. performed research; M.A.M. analyzed data; and M.A.M. and C.L.S. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

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Pubertally born neurons and glia are functional
Margaret A. Mohr, Cheryl L. Sisk
Proceedings of the National Academy of Sciences Mar 2013, 201219443; DOI: 10.1073/pnas.1219443110

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Pubertally born neurons and glia are functional
Margaret A. Mohr, Cheryl L. Sisk
Proceedings of the National Academy of Sciences Mar 2013, 201219443; DOI: 10.1073/pnas.1219443110
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