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Prepaying the entropic cost for allosteric regulation in KIX

Sean M. Law, Jessica K. Gagnon, Anna K. Mapp, and Charles L. Brooks III
PNAS published ahead of print July 7, 2014 https://doi.org/10.1073/pnas.1405831111
Sean M. Law
Departments of aChemistry andbBiophysics, and
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Jessica K. Gagnon
Departments of aChemistry and
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Anna K. Mapp
Departments of aChemistry andcLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109
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Charles L. Brooks
Departments of aChemistry andbBiophysics, and
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  • For correspondence: brookscl@umich.edu
  1. Edited by José N. Onuchic, Rice University, Houston, TX, and approved June 9, 2014 (received for review March 28, 2014)

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    - Aug 19, 2014
  • Allostery within a transcription coactivator is predominantly mediated through dissociation rate constants
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Significance

Understanding how proteins are regulated through the binding of an effector molecule at a nonoverlapping site is a central problem in protein allostery. However, connecting the changes in structural dynamics to changes in the binding affinity can be challenging. Here, we investigate the allosteric mechanism involving a promiscuous protein that serves as a hub for a variety of intrinsically disordered proteins (IDPs). By using a coarse-grained model to simulate the interactions between the protein and two IDPs, we were able to recapitulate experimental binding affinities, capture relevant structural dynamics, and provide a thermodynamic and kinetic description of the allosteric mechanism. It is presumable that the features identified in this allosteric mechanism are conserved between the central protein and other IDPs.

Abstract

The kinase-inducible domain interacting (KIX) domain of the CREB binding protein (CBP) is capable of simultaneously binding two intrinsically disordered transcription factors, such as the mixed-lineage leukemia (MLL) and c-Myb peptides, at isolated interaction sites. In vitro, the affinity for binding c-Myb is approximately doubled when KIX is in complex with MLL, which suggests a positive cooperative binding mechanism, and the affinity for MLL is also slightly increased when KIX is first bound by c-Myb. Expanding the scope of recent NMR and computational studies, we explore the allosteric mechanism at a detailed molecular level that directly connects the microscopic structural dynamics to the macroscopic shift in binding affinities. To this end, we have performed molecular dynamics simulations of free KIX, KIX-c-Myb, MLL-KIX, and MLL-KIX-c-Myb using a topology-based Gō-like model. Our results capture an increase in affinity for the peptide in the allosteric site when KIX is prebound by a complementary effector and both peptides follow an effector-independent folding-and-binding mechanism. More importantly, we discover that MLL binding lowers the entropic cost for c-Myb binding, and vice versa, by stabilizing the L12-G2 loop and the C-terminal region of the α3 helix on KIX. This work demonstrates the importance of entropy in allosteric signaling between promiscuous molecular recognition sites and can inform the rational design of small molecule stabilizers to target important regions of conformationally dynamic proteins.

  • allostery
  • thermodynamics
  • kinetics
  • coupled folding and binding
  • intrinsically disordered proteins

Footnotes

  • ↵1To whom correspondence should be addressed. Email: brookscl{at}umich.edu.
  • Author contributions: S.M.L., A.K.M., and C.L.B. designed research; S.M.L. and J.K.G. performed research; S.M.L. contributed new reagents/analytic tools; S.M.L., J.K.G., A.K.M., and C.L.B. analyzed data; and S.M.L., J.K.G., A.K.M., and C.L.B. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1405831111/-/DCSupplemental.

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Allosteric regulation in KIX
Sean M. Law, Jessica K. Gagnon, Anna K. Mapp, Charles L. Brooks
Proceedings of the National Academy of Sciences Jul 2014, 201405831; DOI: 10.1073/pnas.1405831111

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Allosteric regulation in KIX
Sean M. Law, Jessica K. Gagnon, Anna K. Mapp, Charles L. Brooks
Proceedings of the National Academy of Sciences Jul 2014, 201405831; DOI: 10.1073/pnas.1405831111
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