Supporting Figure 10

Fig. 10. Expression of the TβRI(AAD) or TβRII(ΔCyt) transgene does not alter expression levels of either Neu transgene in bigenic animals. (A) RNase protection analyses using TβRI and neu antisense riboprobes were performed on total RNA isolated from primary breast tumors arising in Neu(YD) monogenic and YD/AAD bigenic animals. (B) RNase protection analyses using TβRII and neu antisense riboprobes was performed on total RNA harvested from several primary breast tumors from Neu(YB) monogenic and YB/ΔCyt bigenic animals. (C) RNase protection analyses using TβRI and neu antisense riboprobes were performed on total RNA isolated from breast tumors from Neu(YB) monogenic and YB/AAD bigenic animals. tRNA served as a negative control. BT refers to breast tumor. (Lower) A mouse-specific β-actin antisense riboprobe was included to control for the amount of RNA in each hybridization reaction. Undig. Riboprobes refers to the full-length riboprobes that have not been subjected to RNase digestion.