Supporting Figure 11

Fig. 11. Neu(YB) and Neu(YD) mammary tumors express all three TGF-β isoforms. RNase protection analyses using mouse-specific TGF-β1, TGF-β2, and TGF-β3 antisense riboprobes were performed on total RNA isolated from Neu(YB) and Neu(YD) breast tumors (BT). The numbers refer to ear tag identification numbers of individual transgenic mice. A mouse-specific β-actin antisense riboprobe was included to control for the amount of RNA in each hybridization reaction. Undig. Riboprobes refers to the full-length riboprobes that have not been subjected to RNase digestion. tRNA alone served as a negative control.