Neuronal migration in the murine rostral migratory stream requires serum response factor
Abstract
The central nervous system is fundamentally dependent on guided cell migration, both during development and in adulthood. We report an absolute requirement of the transcription factor serum response factor (SRF) for neuronal migration in the mouse forebrain. Conditional, late-prenatal deletion of Srf causes neurons to accumulate ectopically at the subventricular zone (SVZ), a prime neurogenic region in the brain. SRF-deficient cells of the SVZ exhibit impaired tangential chain migration along the rostral migratory stream into the olfactory bulb. SVZ explants display retarded chain migration in vitro. Regarding target genes, SRF deficiency impairs expression of the β-actin and gelsolin genes, accompanied by reduced cytoskeletal actin fiber density. At the posttranslational level, cofilin, a key regulator of actin dynamics, displays dramatically elevated inhibitory phosphorylation at Ser-3. Our studies indicate that SRF-controlled gene expression directs both the structure and dynamics of the actin microfilament, thereby determining cell-autonomous neuronal migration.
Acknowledgments
We greatly appreciate excellent technical support by S. Herberich and C. Krüger and useful comments on the manuscript by B. Knöll. We thank W. Witke (European Molecular Biology Laboratory, Monterotondo) for anti-gelsolin antisera and acknowledge insightful discussions with F. Haiss. A.N. was financed by the Deutsche Forschungsgemeinschaft (120/12-1 and SFB446/B7) and the Fonds der Chemischen Industrie. M.F. and O.K. were supported by the Deutsche Forschungsgemeinschaft (SFB505 and TR-3). U.P. received a Ph.D. scholarship from the Boehringer Ingelheim Fonds.
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Copyright © 2005, The National Academy of Sciences.
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Revision received: February 11, 2005
Published online: April 18, 2005
Published in issue: April 26, 2005
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Acknowledgments
We greatly appreciate excellent technical support by S. Herberich and C. Krüger and useful comments on the manuscript by B. Knöll. We thank W. Witke (European Molecular Biology Laboratory, Monterotondo) for anti-gelsolin antisera and acknowledge insightful discussions with F. Haiss. A.N. was financed by the Deutsche Forschungsgemeinschaft (120/12-1 and SFB446/B7) and the Fonds der Chemischen Industrie. M.F. and O.K. were supported by the Deutsche Forschungsgemeinschaft (SFB505 and TR-3). U.P. received a Ph.D. scholarship from the Boehringer Ingelheim Fonds.
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Neuronal migration in the murine rostral migratory stream requires serum response factor, Proc. Natl. Acad. Sci. U.S.A.
102 (17) 6148-6153,
https://doi.org/10.1073/pnas.0501191102
(2005).
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