Biophysical properties of the clinical-stage antibody landscape

Edited by James A. Wells, University of California, San Francisco, CA, and approved December 13, 2016 (received for review October 2, 2016)
January 17, 2017
114 (5) 944-949

Significance

In addition to binding to a desired target molecule, all antibody drugs must also meet a set of criteria regarding the feasibility of their manufacture, stability in storage, and absence of off-target stickiness. This suite of characteristics is often termed “developability.” We present here a comprehensive analysis of these properties for essentially the full set of antibody drugs that have been tested in phase-2 or -3 clinical trials, or are approved by the FDA. Surprisingly, many of the drugs or candidates in this set exhibit properties that indicate significant developability risks; however, the number of such red warning flags decreases with advancement toward approval. This reference dataset should help prioritize future drug candidates for development.

Abstract

Antibodies are a highly successful class of biological drugs, with over 50 such molecules approved for therapeutic use and hundreds more currently in clinical development. Improvements in technology for the discovery and optimization of high-potency antibodies have greatly increased the chances for finding binding molecules with desired biological properties; however, achieving drug-like properties at the same time is an additional requirement that is receiving increased attention. In this work, we attempt to quantify the historical limits of acceptability for multiple biophysical metrics of “developability.” Amino acid sequences from 137 antibodies in advanced clinical stages, including 48 approved for therapeutic use, were collected and used to construct isotype-matched IgG1 antibodies, which were then expressed in mammalian cells. The resulting material for each source antibody was evaluated in a dozen biophysical property assays. The distributions of the observed metrics are used to empirically define boundaries of drug-like behavior that can represent practical guidelines for future antibody drug candidates.

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Acknowledgments

We thank other Adimab LLC staff members for their many contributions.

Supporting Information

Supporting Information (PDF)
Supporting Information
pnas.1616408114.sd01.xlsx
pnas.1616408114.sd02.xlsx
pnas.1616408114.sd03.xlsx

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Information & Authors

Information

Published in

The cover image for PNAS Vol.114; No.5
Proceedings of the National Academy of Sciences
Vol. 114 | No. 5
January 31, 2017
PubMed: 28096333

Classifications

Submission history

Published online: January 17, 2017
Published in issue: January 31, 2017

Keywords

  1. monoclonal antibody
  2. developability
  3. biophysical properties
  4. manufacturability
  5. nonspecificity

Acknowledgments

We thank other Adimab LLC staff members for their many contributions.

Notes

This article is a PNAS Direct Submission.

Authors

Affiliations

Tushar Jain1
Department of Computational Biology, Adimab LLC, Lebanon, NH 03766;
Tingwan Sun1
Department of Protein Analytics, Adimab LLC, Lebanon, NH 03766;
Stéphanie Durand
Department of Molecular Biology, Adimab LLC, Lebanon, NH 03766;
Amy Hall
Department of Molecular Biology, Adimab LLC, Lebanon, NH 03766;
Nga Rewa Houston
Department of Molecular Biology, Adimab LLC, Lebanon, NH 03766;
Department of Antibody Discovery, Adimab LLC, Lebanon, NH 03766;
Juergen H. Nett
Department of High-Throughput Expression, Adimab LLC, Lebanon, NH 03766
Beth Sharkey
Department of High-Throughput Expression, Adimab LLC, Lebanon, NH 03766
Beata Bobrowicz
Department of High-Throughput Expression, Adimab LLC, Lebanon, NH 03766
Isabelle Caffry
Department of Protein Analytics, Adimab LLC, Lebanon, NH 03766;
Yao Yu
Department of Protein Analytics, Adimab LLC, Lebanon, NH 03766;
Yuan Cao
Department of Protein Analytics, Adimab LLC, Lebanon, NH 03766;
Heather Lynaugh
Department of Protein Analytics, Adimab LLC, Lebanon, NH 03766;
Michael Brown
Department of Protein Analytics, Adimab LLC, Lebanon, NH 03766;
Hemanta Baruah
Department of Antibody Discovery, Adimab LLC, Lebanon, NH 03766;
Laura T. Gray
Department of Antibody Discovery, Adimab LLC, Lebanon, NH 03766;
Eric M. Krauland
Department of Antibody Discovery, Adimab LLC, Lebanon, NH 03766;
Department of Protein Analytics, Adimab LLC, Lebanon, NH 03766;
Maximiliano Vásquez2 [email protected]
Department of Computational Biology, Adimab LLC, Lebanon, NH 03766;
K. Dane Wittrup2 [email protected]
Department of Computational Biology, Adimab LLC, Lebanon, NH 03766;
Department of Protein Analytics, Adimab LLC, Lebanon, NH 03766;
Department of Molecular Biology, Adimab LLC, Lebanon, NH 03766;
Department of Antibody Discovery, Adimab LLC, Lebanon, NH 03766;
Department of High-Throughput Expression, Adimab LLC, Lebanon, NH 03766

Notes

2
To whom correspondence may be addressed. Email: [email protected], [email protected], or [email protected].
Author contributions: T.J., E.M.K., Y.X., M.V., and K.D.W. designed research; T.J., T.S., S.D., A.H., N.R.H., J.H.N., B.S., B.B., I.C., Y.Y., Y.C., H.L., M.B., H.B., L.T.G., Y.X., and M.V. performed research; S.D., A.H., N.R.H., J.H.N., B.S., and B.B. contributed new reagents/analytic tools; T.J., T.S., J.H.N., B.S., I.C., Y.Y., Y.C., H.L., M.B., H.B., L.T.G., E.M.K., Y.X., M.V., and K.D.W. analyzed data; and T.J., T.S., J.H.N., E.M.K., Y.X., M.V., and K.D.W. wrote the paper.
1
T.J. and T.S. contributed equally to this work.

Competing Interests

Conflict of interest statement: All of the authors are employed by Adimab, LLC, whose business is the discovery of antibody drugs.

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    Biophysical properties of the clinical-stage antibody landscape
    Proceedings of the National Academy of Sciences
    • Vol. 114
    • No. 5
    • pp. 783-E905

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