Abstract

A method for obtaining strongly polarized nuclear spins in solution has been developed. The method uses low temperature, high magnetic field, and dynamic nuclear polarization (DNP) to strongly polarize nuclear spins in the solid state. The solid sample is subsequently dissolved rapidly in a suitable solvent to create a solution of molecules with hyperpolarized nuclear spins. The polarization is performed in a DNP polarizer, consisting of a super-conducting magnet (3.35 T) and a liquid-helium cooled sample space. The sample is irradiated with microwaves at ≈94 GHz. Subsequent to polarization, the sample is dissolved by an injection system inside the DNP magnet. The dissolution process effectively preserves the nuclear polarization. The resulting hyperpolarized liquid sample can be transferred to a high-resolution NMR spectrometer, where an enhanced NMR signal can be acquired, or it may be used as an agent for in vivo imaging or spectroscopy. In this article we describe the use of the method on aqueous solutions of [13C]urea. Polarizations of 37% for 13C and 7.8% for 15N, respectively, were obtained after the dissolution. These polarizations correspond to an enhancement of 44,400 for 13C and 23,500 for 15N, respectively, compared with thermal equilibrium at 9.4 T and room temperature. The method can be used generally for signal enhancement and reduction of measurement time in liquid-state NMR and opens up for a variety of in vitro and in vivo applications of DNP-enhanced NMR.

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References

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Published in

Go to Proceedings of the National Academy of Sciences
Go to Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences
Vol. 100 | No. 18
September 2, 2003
PubMed: 12930897

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Submission history

Received: April 16, 2003
Published online: August 20, 2003
Published in issue: September 2, 2003

Authors

Affiliations

Jan H. Ardenkjær-Larsen*
Amersham Health Research and Development AB, Medeon, SE-205 12 Malmö, Sweden
Björn Fridlund
Amersham Health Research and Development AB, Medeon, SE-205 12 Malmö, Sweden
Andreas Gram
Amersham Health Research and Development AB, Medeon, SE-205 12 Malmö, Sweden
Georg Hansson
Amersham Health Research and Development AB, Medeon, SE-205 12 Malmö, Sweden
Lennart Hansson
Amersham Health Research and Development AB, Medeon, SE-205 12 Malmö, Sweden
Mathilde H. Lerche
Amersham Health Research and Development AB, Medeon, SE-205 12 Malmö, Sweden
Rolf Servin
Amersham Health Research and Development AB, Medeon, SE-205 12 Malmö, Sweden
Mikkel Thaning
Amersham Health Research and Development AB, Medeon, SE-205 12 Malmö, Sweden
Klaes Golman
Amersham Health Research and Development AB, Medeon, SE-205 12 Malmö, Sweden

Notes

*
To whom correspondence should be addressed. E-mail: [email protected].
Communicated by Albert W. Overhauser, Purdue University, West Lafayette, IN, June 20, 2003

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    Increase in signal-to-noise ratio of > 10,000 times in liquid-state NMR
    Proceedings of the National Academy of Sciences
    • Vol. 100
    • No. 18
    • pp. 10137-10580

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