Directing gene expression to cerebellar granule cells using γ-aminobutyric acid type A receptor α6 subunit transgenes
Abstract
Expression of the γ-aminobutyric acid type A receptor α6 subunit gene is restricted to differentiated granule cells of the cerebellum and cochlear nucleus. The mechanisms underlying this limited expression are unknown. Here we have characterized the expression of a series of α6-based transgenes in adult mouse brain. A DNA fragment containing a 1-kb portion upstream of the start site(s), together with exons 1–8, can direct high-level cerebellar granule cell-specific reporter gene expression. Thus powerful granule cell-specific determinants reside within the 5′ half of the α6 subunit gene body. This intron-containing transgene appears to lack the cochlear nucleus regulatory elements. It therefore provides a cassette to deliver gene products solely to adult cerebellar granule cells.
Acknowledgments
We thank R. Palmiter for the pnLacF vector, A. Lenton for help with figure preparation, and H. Bading, M. Goedert, A. L. Grant, S. P. Hunt, and S. Munro for discussion. G. King, T. Langford, I. Lavenir, and T. Rabbitts provided invaluable support with transgenic mice. S.B. holds a European Community Training and Mobility of Researchers Fellowship (category 20).
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Copyright © 1997, The National Academy of Sciences of the USA.
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Received: April 23, 1997
Accepted: June 6, 1997
Published online: August 19, 1997
Published in issue: August 19, 1997
Acknowledgments
We thank R. Palmiter for the pnLacF vector, A. Lenton for help with figure preparation, and H. Bading, M. Goedert, A. L. Grant, S. P. Hunt, and S. Munro for discussion. G. King, T. Langford, I. Lavenir, and T. Rabbitts provided invaluable support with transgenic mice. S.B. holds a European Community Training and Mobility of Researchers Fellowship (category 20).
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Directing gene expression to cerebellar granule cells using γ-aminobutyric acid type A receptor α6 subunit transgenes, Proc. Natl. Acad. Sci. U.S.A.
94 (17) 9417-9421,
https://doi.org/10.1073/pnas.94.17.9417
(1997).
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