PT  - JOURNAL ARTICLE
AU  - Löfdahl, S
AU  - Guss, B
AU  - Uhlén, M
AU  - Philipson, L
AU  - Lindberg, M
TI  - Gene for staphylococcal protein A
AID  - 10.1073/pnas.80.3.697
DP  - 1983 Feb 01
TA  - Proceedings of the National Academy of Sciences
PG  - 697--701
VI  - 80
IP  - 3
4099  - http://www.pnas.org/content/80/3/697.short
4100  - http://www.pnas.org/content/80/3/697.full
SO  - Proc Natl Acad Sci USA1983 Feb 01; 80
AB  - The gene for protein A from Staphylococcus aureus was cloned into pBR322 in Escherichia coli. An immunoassay was used to detect production of the protein. Protein A produced in E. coli was found in the periplasmic space and was purified and concentrated by IgG-Sepharose affinity chromatography. DNA sequence assay of the gene revealed a region with the general features of a prokaryotic signal peptide and a fifth structural region homologous to the four repetitive regions found earlier by amino acid sequence determination of the mature protein. Upstream from the structural gene there is a possible promoter region and a ribosomal binding sequence typical of gram-positive bacteria. The initiation codon is TTG.