RT Journal Article
SR Electronic
T1 Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage
JF Proceedings of the National Academy of Sciences
JO Proc Natl Acad Sci USA
FD National Academy of Sciences
SP 1921
OP 1926
DO 10.1073/pnas.96.5.1921
VO 96
IS 5
A1 Raderschall, Elke
A1 Golub, Efim I.
A1 Haaf, Thomas
YR 1999
UL http://www.pnas.org/content/96/5/1921.abstract
AB A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After treatment of cells with DNA-damaging agents or γ irradiation, ssDNA molecules form nuclear foci in a dose-dependent manner within 60 min. The mammalian recombination protein Rad51 and the replication protein A then accumulate at sites of ssDNA and form foci, suggesting that these are sites of recombinational DNA repair. ds,double stranded;DSB,double-strand break;FISEL,fluorescence in situ end labeling;MMC,mitomycin C;RPA,replication protein A;ss,single stranded;XPA,Xeroderma pigmentosum group A