PT  - JOURNAL ARTICLE
AU  - Wolfgang, Curt D.
AU  - Essand, Magnus
AU  - Vincent, James J.
AU  - Lee, Byungkook
AU  - Pastan, Ira
TI  - TARP: A nuclear protein expressed in prostate and breast cancer cells derived from an alternate reading frame of the T cell receptor γ chain locus
AID  - 10.1073/pnas.160270597
DP  - 2000 Aug 15
TA  - Proceedings of the National Academy of Sciences
PG  - 9437--9442
VI  - 97
IP  - 17
4099  - http://www.pnas.org/content/97/17/9437.short
4100  - http://www.pnas.org/content/97/17/9437.full
SO  - Proc Natl Acad Sci USA2000 Aug 15; 97
AB  - Previously, we identified the expression of a prostate-specific form of T cell receptor γ chain (TCRγ) mRNA in the human prostate and demonstrated that it originates from epithelial cells and not from infiltrating T lymphocytes. Here, we show that this prostate-specific transcript is also expressed in three breast cancer cell lines and breast cancer tissues. Analysis of the cDNA sequence predicts that this transcript can encode two protein products of 7 and 13 kDa, and in vitro translation experiments showed that both proteins were made. The longer ORF encodes a 13-kDa truncated version of TCRγ, whereas the shorter alternative reading frame encodes a 7-kDa protein with five leucine residues in heptad repeats followed by a basic region. Studies with specific antibodies against each protein product revealed that both prostate and breast cancer cells contain only the 7-kDa protein, which is located in the nucleus. We have named this protein TCRγ alternate reading frame protein (TARP). These results demonstrate that an alternative protein product is encoded by the TCRγ locus in cells other than T lymphocytes. TCRγ,T cell receptor γ chain;TARP,TCRγ alternate reading frame protein;EST,expressed sequence tag;PE,Pseudomonas exotoxin;RT-PCR,reverse transcription–PCR;Tricine,N-tris(hydroxymethyl)methylglycine;Tup,thymidine uptake;ARE,androgen response element