RT Journal Article
SR Electronic
T1 Real-time visualization of intracellular hydrodynamics in single living cells
JF Proceedings of the National Academy of Sciences
JO Proc Natl Acad Sci USA
FD National Academy of Sciences
SP 1577
OP 1582
DO 10.1073/pnas.98.4.1577
VO 98
IS 4
A1 Potma, Eric O.
A1 de Boeij, Wim P.
A1 van Haastert, Peter J. M.
A1 Wiersma, Douwe A.
YR 2001
UL http://www.pnas.org/content/98/4/1577.abstract
AB Intracellular water concentrations in single living cells were visualized by nonlinear coherent anti-Stokes Raman scattering (CARS) microscopy. In combination with isotopic exchange measurements, CARS microscopy allowed the real-time observation of transient intracellular hydrodynamics at a high spatial resolution. Studies of the hydrodynamics in the microorganism Dictyostelium discoideum indicated the presence of a microscopic region near the plasma membrane where the mobility of water molecules is severely restricted. Modeling the transient hydrodynamics eventuated in the determination of cell-specific cytosolic diffusion and plasma membrane permeability constants. Our experiments demonstrate that CARS microscopy offers an invaluable tool for probing single-cell water dynamics. CARS,coherent anti-Stokes Raman scattering;OPO,optical parametric oscillator;ANTS,aminonaphtalene-trisulfonic acid;TPE,two-photon excited