RT Journal Article
SR Electronic
T1 Inositol pyrophosphate mediated pyrophosphorylation of AP3B1 regulates HIV-1 Gag release
JF Proceedings of the National Academy of Sciences
JO Proc Natl Acad Sci USA
FD National Academy of Sciences
SP 21161
OP 21166
DO 10.1073/pnas.0909176106
VO 106
IS 50
A1 Azevedo, Cristina
A1 Burton, Adam
A1 Ruiz-Mateos, Ezequiel
A1 Marsh, Mark
A1 Saiardi, Adolfo
YR 2009
UL http://www.pnas.org/content/106/50/21161.abstract
AB High-energy inositol pyrophosphates, such as IP7 (diphosphoinositol pentakisphosphate), can directly donate a β-phosphate to a prephosphorylated serine residue generating pyrophosphorylated proteins. Here, we show that the β subunit of AP-3, a clathrin-associated protein complex required for HIV-1 release, is a target of IP7-mediated pyrophosphorylation. We have identified Kif3A, a motor protein of the kinesin superfamily, as an AP3B1-binding partner and demonstrate that Kif3A, like the AP-3 complex, is involved in an intracellular process required for HIV-1 Gag release. Importantly, IP7-mediated pyrophosphorylation of AP3B1 modulates the interaction with Kif3A and, as a consequence, affects the release of HIV-1 virus-like particles. This study identifies a cellular process that is regulated by IP7-mediated pyrophosphorylation.