Table S1.

set-2 and set-16 suppress only a subset of the vulval defects associated with the post-L1 diapause aak-1/2 mutants

StarvationEvl, % (95% CI)Burst, % (95% CI)Bag, % (95% CI)Muv, % (95% CI)n
No starvation
Wild-type0 (0–3.2)0 (0–3.2)0 (0–3.2)0 (0–3.2)158
 aak-1/20 (0–1.8)1.5 (0.5–4.2)0 (0–1.9)0 (0–1.9)204
 set-20 (0–3.30 (0–3.3)0 (0–3.3)0 (0–3.3)116
 aak-1/2; set-20 (0–1.7)0.5 (0.1–2.6)0.9 (0.3–3.3)0 (0–1.7)217
Three-day starvation
Wild-type0 (0–1)0 (0–1)0 (0–1)0 (0–1)200
 aak-1/211.8 (8.5–16.1)*13.2 (9.7–17.7)3.9 (2.2–6.9)2.1 (1–4.6)280
 set-20 (0–3.3)0 (0–3.3)0 (0–3.3)0 (0–3.3)111
 aak-1/2; set-22.6 (1.2–5.6)*13.6 (9.8–18.7)0.9 (0.2–3.1)0 (0–1.7)228
Eleven-day starvation
Wild-type0 (0–7.4)0 (0–7.4)25 (14.9–38.8)0 (0–7.4)48
Three-day starved aak-1/2 treated with RNAi by soaking
CTL62.9 (53.9–71.2)8.6 (4.8–15.1)0.9 (0.2–4.7)2.6 (0.9–7.3)116
 set-2 (RNAi)41.3 (32.4–51)12.5 (7.5–20.2)1 (0.2–5.24)1.9 (9.5–6.7)104
 set-16 (RNAi)44 (33.5–55.3)16 (9.2–25.9)0 (0–4.902.7 (0.7–9.2)75
 wdr-5 (RNAi)49.4 (38.9–59.9)15.7 (9.4–25)0 (0–4.4)1.2 (0.2–6.5)83
 ash-2 (RNAi)49.4 (38.9–59.9)8.4 (4.1–16.4)2.4 (0.7–8.4)2.4 (0.7–8.4)83
  • Animals of indicated genotype were starved (–, 3, or 11 d), singled to replete plates, and scored for vulva or gonadal phenotypes when they reached the adult stage. The set-2 allele [set-2(ok952)] is a weak hypomorph. For the RNAi experiments, aak-1/2 mutants were treated by soaking L1 larvae in buffer containing dsRNA that corresponded to COMPASS complex components during the L1 diapause. Animals were then recovered to replete plates, singled, and scored for vulva or gonadal phenotypes when they reached the adult stage. Control animals (CTL) are aak-1/2 L1 larvae maintained 3-d in M9 buffer containing GFP dsRNA. Results of three independent post-L1 diapause recovery experiments were pooled. Parenthesis indicate 95% CI. Letters indicate significant differences between two conditions (P < 0.0005) by Fisher’s exact test.

  • * The frequency of Evl animals observed is significantly different between aak-1/2 and aak-1/2; set-2 mutant larvae.

  • The frequency of Evl animals observed is significantly different between aak-1/2 (CTL) and aak-1/2; set-2 (RNAi) larvae.

  • The frequency of Evl animals observed is significantly different between aak-1/2 (CTL) and aak-1/2; set-16 (RNAi) larvae.