Table 1

E. coli strains and plasmids

Strain or plasmidGenotype and phenotypeSource
Strains
 χ289E. coli K-12 W1485 λ, FglnV44Ref. 11
 χ7122Avian pathogenic, O78:K80:H9, gyrA, NalRRef. 23
 χ7273tsh∷tetAR(B), TcR (Tsh)χ7122; ref. 18
 χ7301ΔiucABCD iutA∷xylE tsh∷tetAR(B) TcR (Aerobactin, Tsh)χ7273
 χ7303ΔiroBCDEN∷nptII, KmR (Iro)χ7122
 χ7306ΔiroBCDEN∷nptII tsh∷tetAR(B) ΔiucABCDiutA∷xylE TcR, KmR (Tsh, Iro, Aerobactin)χ7301
Plasmids
 pACYC184p15A replicon, TcR, CmRRef. 24
 pBSL86nptII cassette vector. KmR, ApRRef. 25
 pC6E. coli rrnB in pBR322, ApRC. Squires
 pMEG-375sacRB mobRP4 oriR6K. CmR, ApRMegan Health (St. Louis, MO)
 pMEG-685xylE cassette vector, ApRMegan Health
 pYA3661pACYC184∷iroBCDEN, CmRThis work
 pYA3662pMEG-375∷ΔiucABCDiutA∷xylE. CmR, ApRThis work
 pYA3663pMEG-375∷ΔiroBCDEN∷nptII. CmR, ApR, KmRThis work
  • Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin; Nal, nalidixic acid; Tc, tetracycline.