Table 3. M. tuberculosis genes with high probabilities of being essential
MT no. Rv no. Gene description Probability, %
0417 0404 Acyl-CoA synthase (fadD30) 83
2062 2006 Trehalose-6-phosphatase 83
3285 3193c Probable integral membrane protein 83
2082 2024c Conserved hypothetical protein 82
3974 3859c Glutamate synthase (gltB) 82
1587 1536 Isoleucyl-tRNA synthase 81
1198 1161 Nitrate reductase[α] subunit (narG) 79
0047 0041 Leucyl-tRNA synthase (leuS) 79
2600 2524c Fatty acid synthase (Fasl) 78
0070 0064 Probable membrane protein 77
1678 1640c C-term Lysyl-tRNA synthase (lysX) 76
2551 2476c Conserved hypothetical protein 75
1796 1753c PPE-family protein 75
0116 0107c Probable Mg transport ATPase 75
3045 2967c Pyruvate carboxylase 75
  • Percentage probability estimate for each gene is determined by using the 5′80%-3′100-bp rule. MT and Rv numbers refer to the annotated gene numbers in M. tuberculosis CDC 1551 and H37Rv (3, 12)