Table 1. Cell phenotype and susceptibility to HIV pseudotype infection
Infectivity of pseudotypes expressing env gps* Cell-surface expression
Cell line Cell type No env SF162 VSV G H E1E2 Con1 E1E2 CD81 LDLR SR-B1
Hos.CD4.R5 Human osteosarcoma - ++ +++ - - +++ + +
Huh-7.5 Human hepatoma - - +++ ++ ++ ++ + +
Huh-7 Human hepatoma - - +++ ++ ++ ++ + +
HepG2 Human hepatoma - - +++ - - - + +
PLC/PR5 Human hepatoma - - ++ + + ++ + -
THLE Human liver epithelial - - +++ - - +++ - -
Hela Human epithelial - - ++ - - +++ - +
SW13 Human colorectal adenocarcinoma - - +++ - - +++ + +
U937 Human monocytoid - - + - - - - -
U937-humCD81 Human monocytoid - - + - - +++ - -
THP Human monocytoid - - ++ - - +++ - ++
THP-DC-SIGN Human monocytoid - - ++ - - +++ - ++
RBL Rat basophil - - ++ - - - - -
RBL-humCD81 Rat basophil - - ++ - - +++ - -
  • Virus pseudotypes with no env, H E1E2, Con1 E1E2, and SF162 were tested for infection of the various cell lines at 5 and 1 ng per well (data not shown) and VSV G at 0.1 ng per well

  • * Infectivity is measured as RLU where: -, <300; +, 10,000-20,000; ++, >20,000-100,000; and +++, >100,000

  • Cell-surface expression of antigens, where an irrelevant isotype-matched IgG gave mean fluorescence intensity (MFI) in the range of 4-6 and specific mAbs gave staining intensities of +, 10-30; ++, >30-100; and +++, >100