Table 2. Flow cytometric analysis of selected T cell surface markers before and after two treatment cycles
HLA-DR+ CD45RO+ CD69+ CD4+CD25+ CD25+ CTLA-4+
Patient Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post
% of CD3+CD4+ % of CD3+CD4+ % of CD3+CD4+ % of CD3+ % of CD4+ % of CD4+CD25+
1 17.5 41.8 85.4 88.9 4.6 4.3 15.9 11.4 41.8 35.0 0.4 2.5
3 5.2 17.3 78.8 82.2 1.7 3.4 19.5 10.7 34.6 26.7 3.0 1.1
5 17.6 29.2 77.2 84.6 2.8 1.5 19.0 18.2 42.3 36.4 2.0 1.1
6 31.3 42.5 98.9 99.0 4.4 3.1 14.3 13.5 46.3 35.8 1.5 0.6
7 6.2 20.3 57.0 72.9 1.4 2.0 14.1 23.8 26.7 40.8 0.4 2.4
10 9.8 22.7 85.7 94.3 2.4 3.6 23.4 19.4 46.5 35.5 0.2 1.7
11 6.2 12.2 95.2 89.6 9.8 1.7 21.2 17.6 43.3 25.4 0.0 0.6
12 3.9 31.7 73.7 76.4 1.5 3.9 10.1 11.1 19.9 26.0 0.2 5.5
14 7.9 39.4 72.4 89.4 2.3 6.1 12.8 7.0 28.9 20.5 0.6 2.5
Mean Δ ± SEM +16.8 ± 2.9 (P = 0.0004) +5.9 ± 2.4 (P = 0.04) -0.1 ± 1.1 (P = 0.9) -1.9 ± 1.8 (P = 0.3) -5.4 ± 3.2 (P = 0.13) +1.1 ± 0.7 (P = 0.17)
% of CD3+CD4-%of CD3+CD4-% of CD3+CD4-
130.557.966.169.07.76.8
314.822.352.458.83.63.9
528.931.353.855.84.32.7
678.067.451.361.412.58.2
712.313.352.943.76.72.5
1029.644.380.282.02.17.8
114.918.277.670.48.17.4
128.435.869.672.13.88.9
1414.120.757.960.97.07.6
Mean Δ ± SEM +10.0 ± 4.1 (P = 0.04) +1.4 ± 2.0 (P = 0.51) 0.0 ± 1.2 (P = 1.0)
  • Values compared by using paired t test