Table 1. Activation of LEE1 transcription and bioluminescence in V. harveyi by intestinal flora
Media LEE1::lacZ transcription* Luminescence V. harveyi BB170
SHIME 239 ± 23 1 ± 0.1
PC§-SHIME-flora 2,910 ± 100 38 ± 3.5
PC-86-24 1,367 ± 209 78 ± 20
PC-DH5α 220 ± 20 1.5 ± 0.1
  • Expressed in fold-induction of bioluminescence compared with media alone.

    SHIME media from the artificial intestine (Supporting Text)

  • * LEE1::lacZ transcription is expressed in Miller units of β-galactosidase.

  • BB170 is a V. harveyi luxN::Tn5 that only produces light in response to AI-2.

  • § PC, media preconditioned with the intestinal flora, EHEC strain 86-24 (which produces AI-2), or K-12 strain DH5α (which does not produce AI-2).