Table 1. Activity of mutant sGC in intact Sf9 cells or neuroblastoma cell lysates
Sf9 with mutant* Sf9 with wild type* BE2/pCDNA3.1 βCys-105 BE2/pCDNA3.1 vector
Basal NO Basal NO Basal NO Basal NO
cGMP levels, nmol/mg of protein 35.0 ± 1.59 35.8 ± 1.42 0.4 ± 0.02 4.9 ± 0.13
sGC activity, pmol/min per mg of protein 60.0 ± 12.1 150.2 ± 8.2 <5 300.4 ± 15.9
  • Values are means ± SD of three measurements.

  • * cGMP levels 48 h postinfection for αβCys-105 and 72 h postinfection for αβ sGC.

  • 250 μM NO donor spermine NONOate was used for intact Sf9 cells and 100 μM SNP was used for BE2 lysates.

  • The activity is at or lower than the detection level for this method.