Table 2. Absorption spectra of various forms of native and altered hemoproteins
n.u.v. Soret β α Ref.
Wild-type sGC
    Ferric 393 14, 27
    Ferrous (native) 431 560-590 560-590 27
    Ferrous + CO 423 541 567 14
    Ferrous + NO 399 537 572 14, 27
Reconstituted αβCys-105 sGC
    Ferric 365 417 542 573 This study
    Ferrous ND 426 540 This study
    Ferrous + CO ND 421 536 567 This study
    Ferrous + NO 399 567 611 This study
Cytochrome P450
    Ferric 393 645 39, 40
    Ferrous 411 542 39, 40
    Ferrous + CO 366 447 39, 40
Cytochrome P420
    Ferric 366 418 540 570 39, 40
    Ferrous 325 428 535 562 39, 40
    Ferrous + CO 341 423 541 570 39, 40
  • Values for the αβCys-105 mutant are reported. n.u.v., near-UV band; ND, not detected because of the overlapping dithionate absorption but absent when DTT was used (data not shown).