Table 1

Comparison of predicted and known substrate specificities for selected protein kinases

Protein kinasePredicted optimal heptapeptide substrate sequence*Optimal sequence based on oriented peptide library experimentsConsensus of known substrates
Protein kinases used to develop the rules
 PKA(RK)(RKS)X(ST)(FLMI)(AVI)(ILMARK)(RH)(RKH)(RV)S(IFM)(IVF)(FIM) (1)(RK)(RK)(SLRGAP)(ST)(LSVR)(STPV)(SAVGE) (20)
 PKCα(RKQH)(RKM)X(ST)(FLY)(RKHQ)(AVFLRK)(RK)(KQR)GS(FMIV)(KRF)(KFR) (16)(RKS)(RKAG)(LSAR)(ST)(FLVRK)(RKAS)(RKS) (20)
 PKCζ(RF)(RKM)X(ST)(FLV)(RKHQ)(AVFLRK)R(QKY)(GKM)S(FM)(FMA)(YK) (16)RQGSFFA (16)
 CaMKII(RK)(QMRFST)X(ST)(FLY)(EDS)(LIM)(RQ)(QM)(QMK)S(FIML)(DEI)(LIMK) (15)(RKG)(QARKLS)(AQLG)(ST)(VLIF)(SADG)(SEMD) (20)
 PKCμ(RK)(QMTAS)X(ST)(VILMF)(AVI)(ALIMPFRK)R(QKEM)(MLK)S(VML)(AV)(FY) (16)RTASVAF (16)
 PHK(RK)(QRMS)X(ST)(VILMF)(QNRKH)(ALIMPFRKHQ)(RK)(MRQF)(MFL)S(FIM)(LI)(FLI) (15)R(AQSTL)(ILR)(ST)(VITA)(RHY)(RKFS) (20)
 NIMA(RF)(RV)X(ST)R(EDS)(AVFLRK)(FLM)(RK)(RK)S(RIVM)(RIMV)(MIFV) (15)FRSSIRR (15)
 CK2(RKQHDSE)(EDS)X(ST)E(DE)(EDS)(DE)(ED)(ED)S(EDA)(EA)(EA) (15)(DSE)(ESD)(EGS)(ST)(DE)(ED)E (20)
 CDK2(NGSL)(PVALS)X(ST)P(KRMI)(RKQSL)H(PH)RSPRK (1)(TL)(PVLSH)(LATS)(ST)P(PR)(KRL) (20)
Protein kinases with peptide library information, used to test predictions
 PKCγ(RKQH)(RKM)X(ST)(FLY)(RKHQ)(AVFLRK)R(KRQ)(GK)S(FKM)(KR)(RK) (16)
 PKCδ(RF)(RKM)X(ST)(FLV)(RKHQ)(AVFLRK)R(KRQ)(GA)S(FMV)(FKM)Y (16)MRQSVAV (16)
 AKT(RK)(RKS)X(ST)(FLV)(STNGAP)(AVFLRK)RT(YFGM)S(FM)(GTS) (17)R(RTSL)(SPRT)(ST)(YSF)(PGAST)(EAND) (19)
 Slk1 (Bck1)(QNRKH)(VSAR)X(ST)(AVILMY)(SQN)(AVLIFRK)(RK)(FR)(GR)S(LIFM)(RIMF)(RFM) (1)(LA)(AV)X(ST)(FL)(TA)(TG) (1)
 Chk1(RK)(SALF)X(ST)(VA)(AVI)(MFLIRK)R(YMP)(RF)S(FINM)(SA)(IFL) (19)RSPSMPE (19)
 Chk2(RK)(ILMA)X(ST)(FLMID)(QNRKH)(ILMARK)R(YKA)(YE)S(FI)(FIR)(YRF) (19)R(STI)(FHKP)(ST)(DFM)(LVPS)(WLKE) (19)
 CK1γ(QNRKH)(SED)X(ST)(VAILMY)(SQN)(ILMARK)E(TAG)GSI(IYF)(FY) (15)(SDET)(SEAV)(SLDE)(ST)(ELSVI)(ESTD)(ESGD) (20)
 CK1δ(QNRKH)(SED)X(ST)(VAILMY)(QNRKH)(AVLIMPFTSQN)E(TAG)GSI(IFY)(IGY) (15)(SDET)(SEAV)(SLDE)(ST)(ELSVI)(ESTD)(ESGD) (20)
 ERK1(RKF)(PVALS)X(ST)P(PFLI)(RKQSL)(GPE)(PLI)(LMP)SP(GPF)(PFG) (15)(PAVTL)(PV)(LT)(ST)P(PSR)(PARKFG) (15)
 CDK5(NGSL)(PVALS)X(ST)P(KRMI)(RKQSL)H(HP(KG)SP(KR)(HRK) (15)(EG)(TVH)(KA)(ST)P(VPE)K (15)
Protein kinases without peptide library information, used to test predictions
 PKG(RK)(RKS)X(ST)(AVIP)(AVI)(AVFLRK)R(RK)(RLI)(ST)(RASIK)(SALK)(EPT) (20)
 βARK(RKQHE)(QMRFSTY)X(ST)(EQT)(DE)(QNRKHVLIMF)(NTK)(NVT)(DS)(ST)(ENDQ)(EDNG)(RQEDN) (18)
 S6K(1)(RK)(RKS)X(ST)(VAIL)(VLIMFC)(AVFLRK)R(RAS)(LS)(ST)(SVL)(SRL)(SRAG) (20)
 S6K(2)(RKQH)(RKS)X(ST)(AVILMY)(EDS)(AVFLRK)R(LTA)(SL)(ST)(LVH)(SR)(SA) (18)
 CaMKI(RK)(QMRFST)X(ST)(VA)(STNDH)(RK)RRLSDSN (18)
 AMPK(RK)(RQMS)X(ST)(VA)(AGVLIMPFTC)(QNRKH)R(SNT)(MEQN)(ST)(FIGK)(LAI)(HFLA) (18)
  • “−”, not reported. 

  • * X, any amino acid.