Table 4. Gene-trap vector insertion site preference in full-length cDNAs (according to RefSeq)
Gene trap vector Total no. of insertions Insertions in 5′ UTR (%) Insertions in 5′ CDS (%) Insertions in 3′ CDS (%) Insertions in 3′ UTR (%)
pT1βgeo 1,385 222 (16.0) 589 (42.5) 511 (36.9) 63 (4.6)
pT1ATGβgeo 395 97 (24.6) 160 (40.5) 125 (31.6) 13 (3.3)
U3βgeo 324 176 (54.3) 101 (31.2) 31 (9.6) 16 (4.9)
ROSAβgeo 302 100 (33.1) 152 (50.3) 37 (12.3) 13 (4.3)
Total 2,406 595 (24.7) 1,002 (41.6) 704 (29.3) 105 (4.4)
  • 5′ CDS, first half of coding sequence; 3′ CDS, second half of coding sequence.