Table 1. Strains and plasmids used in this study
Strain or plasmid Genotype or markers; characteristics and uses Source or ref.
E. coli strains
    DH10β Host for E. coli cloning; F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 Δ(codB-lacI)3 deoR recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ-rpsL(StrR) Life Technology
    UQ950 E. coli DH5α λ(pir) host for cloning; F-Δ(argF-lac)169 Φ80dlacZ58(ΔM15) glnV44(AS) rfbD1 gyrA96(NalR) recA1 endA1 spoT1 thi-1 hsdR17 deoR λpir+ D. Lies, Caltech
    WM3064 Donor strain for conjugation: thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir(wt)] W. Metcalf, Univ. of Illinois, Urbana
Shewanella strains
    ANA-3 Isolated from an As-treated wooden pier pilling in a brackish estuary (Eel Pond, Woods Hole, MA); Contains arsDABC; respires on As(V); resistant to > 5 mM arsenite. 8
    ARRA3 Shewanella sp. str. ANA-3, ΔarrA3; does not respire As(V) This study
    ARRB1 Shewanella sp. str. ANA-3, ΔarrB1; does not respire As(V) This study
Plasmids/vectors
    pSALT1 pLAFR5-based 45 kb As(III)r cosmid from ANA-3 genomic DNA library; contains arsDABC, Tcr, confers resistance to As(III) and As(V). 8
    pSMV10 9.1-kb mobilizable suicide vector; oriR6K, mobRP4, sacB, Kmr Gmr D. Lies, Caltech
    pΔarrA3 2-kb fusion PCR fragment containing ΔarrA cloned into the Spel site of pSMV10; used to make the ARRA3 ΔarrA strain. This study
    pΔarrBI 2-kb fusion PCR fragment containing ΔarrB cloned into the Spel site of pSMV10; used to make the ARRBI ΔarrB strain. This study
    pBBR1MCS-2 5.1-kb broad-host range plasmid: Kmr, lacZ 15
    parrA4 arrA4 PCR fragment, including the promoter region, cloned into the Spel site of pBBR1MCS-2 This study
    parrB1 arrB1 PCR fragment cloned into the Xhol/Spel sites of pBBR1MCS-2 This study