Table 4. Enrichment of defective cccDNA after clearance
Assayed* cccDNA per cell
Animal Weeks p.i. Cells WT Defective WT Defective Fraction Defective
WC377 4 20 94 2 4.7 0.1 0.02
16 6,300 17 9 0.0027 0.0014 0.34
WC378 4 9 61 4 6.6 0.4 0.04
16 10,800 31 27 0.0029 0.0025 0.46
WC380 4 44 115 3 2.6 0.07 0.03
16 36,400 90 52 0.0025 0.0014 0.36
  • * cccDNA from a known number of cells was analyzed. The number of PCR products migrating at 3 kbp (WT) and the number migrating at <3 kbp (defective) are shown.

  • Calculated.