Table 1. Analysis of mutants in previously uncharacterized HCMV ORFs
ORF Function* Position Plaque Growth
UL21a A 26626, 26696-26842§ Small ≥50
UL23 NE 28101, 28372 Normal Normal
UL24 NE 29987, 29234 Normal Normal
UL25 NE 31051 Normal Normal
UL26 A 32260-32746§, 32608-32639§ Small ≥50
UL27 A 33373, Small ≥50
34360 Normal <50
UL28 A 35138, 35568-35780§ Small <50
Small
UL29 A 36662, 36866-36896§ Small ≥50
UL30 A 37369-37400§ Small ≥50
UL31 NE 38502-38603§ Normal ND
UL32 E 42665 None ND
UL34 E 45188, 44791-44857§ None ND
UL35 NE 46366-46490§ Normal Normal
UL37 E 52698-53091§ None ND
UL38 A 51331, 51131-52126§ Small ≥50
UL44 E 55839, 55982 None ND
UL46 E 59823 None ND
UL48 A 63917, 64165 V. small >104
UL48.5 E 70346 None ND
UL49 E 71412, 70658 None ND
UL50 E 72376, 72886 None ND
UL51 E 73343 None ND
UL52 E 74965, 74547 None ND
UL53 E 76432, 76371 None ND
UL54 E 78776, 78300 None ND
UL56 E 84734, 85868 None ND
UL57 E 88931, 89476 None ND
UL60 E 92405 None ND
UL61 E 93220, 92848 None ND
UL65 NE 96316-96479§ Normal ND
UL70 E 100690, 100598 None ND
UL71 A 103867, 103891 V. small >104
UL72 A 105358, 105294 Normal <50
UL73 E 105923, 105974-106038§ None ND
UL76 A 110832 Small ≥50
UL77 E 111817, 112039 None ND
UL78 NE 113804, 113432 Normal Normal
UL79 E 114597, 114885 None ND
UL80 E 115482, 115511 None ND
UL80/UL80a E 116225 None ND
UL84 E 122829, 122419 None ND
UL85 E 123381, 123207 None ND
UL86 E 125741, 125349 None ND
UL87 E 129657, 129882 None ND
UL88 NE 131675, 131710 Normal Normal
UL89 E 137636, 133118 None ND
UL91 E 133888, 133848-133863§ None ND
UL92 E 134210, 134193 None ND
UL93 E 134841, 134779 None ND
UL94 A 136593, 136650 V. small >104
UL95 E 139394, 138943 None ND
UL96 A 140067, 140182 V. small >104
UL98 E 142723, 143720 None ND
UL99 E 144775**, 144537 None ND
UL102 E 146992, 148532 None ND
UL103 A 149828-149929§, 149717-149759§ V. small >104
UL104 E 151356, 151117 None ND
UL105 E 154266, 152821 None ND
UL111a NE 159730, 160030 Normal Normal
UL112/113 A 160832, 161691-161748§ V. small >104
UL116 NE 165263-165313§ Normal ND
UL117 A 166495 Small ≥50
UL121 NE 168888, 168803 Normal ND
US16 NE 204664-205080§ Normal Normal
US17 NE 206104, 205845-206106§ Normal ND
US18 NE 206740-207198§ Normal Normal
US19 NE 207357, 207708-208018§ Normal ND
US20 NE 208546-208924§, 208665-208870§ Normal Normal
US21 NE 209606-309645§ Normal Normal
US22 NE 210759, 210852 Normal Normal
US23 A 212967-213419§, 212763-212805§ Normal <50
US24 A 213931, 214655-214733§ Small ≥50
US25 NE 215362-215585§ Normal Normal
US26 A 215941, 216346-216665§ Small ≥50
US29 NE 220459-220878§ Normal ND
US30 NE 221894-221923§ Normal ND
US31 NE 222784-222854§ Normal ND
US32 NE 223418-223885§ Normal ND
US34 NE 224483-224920§ Normal ND
US34a NE 225027-225162§ Normal ND
  • * E, essential; NE, nonessential; A, augmenting

  • Plaque size was measured. None, no plaques found after BAC transfection; V. small, very small

  • Multistep growth analysis was performed if a mutant produced small plaques or produced yields at least 10-fold lower than wild type. Numbers indicate folds of growth reduction compared to wild type. ND, not determined

  • § Mutation constructed by site-directed mutagenesis

  • A, tentative assignment

  • Revertant made that grows like wild-type virus

  • ** The transposon insertion truncates one-third of the C terminus of UL99, and the mutant still replicates efficiently