Table 1. Predicted functional classifications of genes in this study
In vivoIn vitro
Functional classification No. of genes Percent of category No. of genes Percent of category
Lipid metabolism 15 7.5 30 14.9
Carbohydrate transport and metabolism 9 8.4 24 22.4
Inorganic ion transport and metabolism 8 8.0 8 8.0
Cell envelope biogenesis, outer membrane 8 7.3 32 29.4
Amino acid transport and metabolism 8 4.3 80 43.0
Transcription 7 5.4 15 11.6
Coenzyme metabolism 7 6.0 38 32.8
DNA replication, recombination and repair 5 4.6 19 17.4
Translation, ribosomal structure 5 3.9 76 59.4
Signal transduction mechanisms 4 5.2 12 15.6
Secretion 3 13.6 8 36.4
Energy production and conversion 3 1.6 31 16.3
Cell division and chromosome partitioning 2 8.7 8 34.8
Posttranslational modification, chaperones 2 2.5 27 34.2
Nucleotide transport and metabolism 1 1.5 25 38.5
Unknown 107 4.7 181 8.0
Total 194 5.0 614 15.7
  • In vivo and in vitro refer to genes that are required for optimal growth under either condition. In vitro genes were identified previously (4). “Percent of category” refers to the fraction of genes of particular functional class that are important for growth under each condition.