Table 3. Estimates of mean selective constraint in intron sequences
Intronic DNA data set Constraint
5′ bases 1-2 1.0 (0.0)
5′ bases 3-6 0.57 (0.044)
5′ bases 7-10 0.025 (0.076)
3′ bases 1-2 1.0 (0.0)
3′ bases 3-16 0.31 (0.031)
3′ bases 17-30 0.15 (0.040)
Intron 1, 5′ end 0.10 (0.017)
Intron 1, 3′ end 0.0056 (0.016)
  • In the analysis of intron 1, up to 6,000 bp at the 3′ or 5′ ends were analyzed. If a first intron was <12,000 bp long, the intron sequence was divided equally at the central nucleotide between data sets of 5′ and 3′ sequences. SEMs are shown in parentheses.